Dillingham M A, Better O S, Anderson R J
Department of Medicine, Veterans Administration Medical Center, Denver, Colorado.
Kidney Int. 1988 Apr;33(4):782-6. doi: 10.1038/ki.1988.67.
Abnormalities in renal tubular function occur commonly in the setting of hyperbilirubinemia. In the present studies, the possibility that the bile salt sodium taurocholate exerts a direct effect on renal epithelial transport was investigated in vitro. We found that addition of sodium taurocholate to fluid bathing perfused rabbit cortical collecting tubules increases hydraulic conductivity (Lp) in a manner similar in time course and significantly less in magnitude to that observed with maximal concentrations of arginine vasopressin. Sodium taurocholate reversibly potentiates rabbit cortical collecting tubular Lp stimulated by maximal concentrations of arginine vasopressin and a nonhydrolyzable cyclic AMP analogue. We also found that sodium taurocholate induces a dose-dependent leak from tubular lumen to bathing fluid of a small molecular weight substance (125I-iothalamate). Our studies suggest that sodium taurocholate increases rabbit cortical collecting tubular Lp by a cyclic AMP independent mechanism.
肾小管功能异常在高胆红素血症情况下很常见。在本研究中，体外研究了牛磺胆酸钠盐对肾上皮转运产生直接影响的可能性。我们发现，将牛磺胆酸钠添加到灌注兔皮质集合管的浴液中，会增加水导率（Lp），其时间进程与用最大浓度精氨酸加压素观察到的相似，但幅度明显较小。牛磺胆酸钠可逆地增强最大浓度精氨酸加压素和一种不可水解的环磷酸腺苷类似物刺激的兔皮质集合管Lp。我们还发现，牛磺胆酸钠诱导一种小分子量物质（125I-碘他拉酸盐）从管腔到浴液的剂量依赖性渗漏。我们的研究表明，牛磺胆酸钠通过一种不依赖环磷酸腺苷的机制增加兔皮质集合管Lp。
