Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran.
Weill Cornell Medicine Qatar, Doha, Qatar.
Adv Exp Med Biol. 2021;1308:273-281. doi: 10.1007/978-3-030-64872-5_19.
Crocin has been shown to have potent antioxidant properties, but its potential antioxidative effects on testicular tissue during uncontrolled diabetes is unknown. Wistar rats were randomly divided into four separate groups; normal, normal-treated, diabetic and diabetic treated (n = 6 per group). Diabetes was induced by a single intravenous injection of streptozotocin (45 mg/kg). Two treated groups of animals (diabetic and non-diabetic) received Crocin daily for 56 days (40 mg/kg/intraperitoneally). At the end of the 56th day, animals were sacrificed and blood and testicular tissue obtained. The level of nitrate, malondialdehyde, glutathione, and the activities of superoxide dismutase and catalase enzymes were determined. Crocin therapy moderated the increased oxidative stress in testicular tissue induced by diabetes with a significant reduction in nitrate and malondialdehyde, whilst reducing superoxide dismutase and catalase enzyme activities in diabetes (p < 0.001), though glutathione was unaffected. Treatment by Crocin in normal rats also modestly improved parameters of oxidative stress (p < 0.05). Crocin has a protective effect on diabetes induced oxidative stress in testicular tissue in an animal model, though it is unclear if this is a direct antioxidant effect.
西红花酸具有很强的抗氧化特性，但它在不受控制的糖尿病期间对睾丸组织的潜在抗氧化作用尚不清楚。Wistar 大鼠被随机分为四组：正常组、正常治疗组、糖尿病组和糖尿病治疗组（每组 6 只）。糖尿病通过单次静脉注射链脲佐菌素（45mg/kg）诱导。两组治疗动物（糖尿病和非糖尿病）每天接受西红花酸治疗 56 天（腹腔内 40mg/kg）。在第 56 天结束时，处死动物并获取血液和睾丸组织。测定硝酸盐、丙二醛、谷胱甘肽以及超氧化物歧化酶和过氧化氢酶活性的水平。西红花酸治疗可减轻糖尿病引起的睾丸组织氧化应激，使硝酸盐和丙二醛显著降低，同时降低糖尿病中超氧化物歧化酶和过氧化氢酶的活性（p<0.001），尽管谷胱甘肽不受影响。在正常大鼠中，西红花酸治疗也适度改善了氧化应激参数（p<0.05）。西红花酸对动物模型中糖尿病引起的睾丸组织氧化应激具有保护作用，但尚不清楚这是否是直接的抗氧化作用。
