State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.
Mol Nutr Food Res. 2021 Jun;65(12):e2100110. doi: 10.1002/mnfr.202100110. Epub 2021 May 10.
Using metabolomics to study the relations of nutrition and health requires stringent control of the experimental conditions used in an animal model. This work investigates the diet effects of autoclaved and irradiated feed on mouse urine and fecal metabolomics.
C57BL/6 mice are fed normal-irradiation sterilized diet (n = 9), autoclave sterilized diet (n = 9), and high-irradiation sterilized diet (n = 9) for 4 weeks. Differential chemical isotope labeling liquid chromatography mass spectrometry is used to quantify the metabolome variations of urine and feces collected at five time points. Significant differences are observed in urine or fecal metabolomes of mice fed autoclaved diet versus mice fed high-irradiation diet or fed normal-irradiation diet, while the differences are small between the mice fed normal-irradiation and high-irradiation diet. Correlation studies of metabolite changes of diet- and aging-related biomarkers indicate a large overlap of significantly affected metabolites by the two factors.
Diet can be a confounding factor that needs to be carefully considered when a metabolomics study is designed and metabolomic results of a mouse model of nutritional or other biological study are interpreted. Using the same sterilized diet for a given metabolomics project is essential to control the diet effect.
使用代谢组学研究营养与健康的关系需要严格控制动物模型中使用的实验条件。本研究调查了高压灭菌和辐照饲料对小鼠尿液和粪便代谢组学的饮食影响。
将 C57BL/6 小鼠分别用常规辐照消毒饮食（n=9）、高压灭菌消毒饮食（n=9）和高辐照消毒饮食（n=9）喂养 4 周。使用差异化学同位素标记液相色谱质谱法对在五个时间点收集的尿液和粪便中的代谢组进行定量分析。与正常辐照饮食组或高辐照饮食组相比，用高压灭菌饮食喂养的小鼠的尿液或粪便代谢组存在显著差异，而用正常辐照饮食和高辐照饮食喂养的小鼠之间差异较小。饮食和衰老相关生物标志物的代谢物变化的相关性研究表明，这两个因素显著影响的代谢物有很大的重叠。
在设计代谢组学研究和解释营养或其他生物学研究的小鼠模型的代谢组学结果时，饮食可能是一个需要仔细考虑的混杂因素。对于给定的代谢组学项目，使用相同的消毒饮食对于控制饮食效果至关重要。
