School of Engineering, RMIT University, Melbourne, Victoria 3001, Australia.
Langmuir. 2021 Apr 27;37(16):4984-4996. doi: 10.1021/acs.langmuir.1c00411. Epub 2021 Apr 16.
Hydroxyapatite (HA) coating has received significant attention in the scientific community for the development of implants, and HA coating on titanium oxide (TiO) nanotubes has shown potential benefits in the improvement of cell proliferation, adhesion, and differentiation. In this study, a HA coating on a TiO nanotubular surface was developed to improve the biocompatibility of the titanium (Ti) surface via magnetron sputtering. Scanning electron microscopy (SEM), surface profilometry, and water contact goniometry revealed that HA-coated TiO nanotubes influenced the surface roughness () and hydrophilicity. The XRD and FTIR peaks indicated the presence of crystalline phases of TiO (anatase) and HA-coated TiO nanotubes after annealing at 500 °C for 120 min. The HA-coated TiO nanotubes showed significantly increased and decreased water contact angle (θ) compared to the as-anodized TiO nanotubular and bare CP-Ti surfaces. MTS assay using osteoblast-like cells confirmed that the HA-coated TiO nanotubular surface provided an enhanced cell attachment and growth when compared to as-anodized TiO nanotubular and pure CP-Ti surfaces.
羟基磷灰石（HA）涂层在植入物的开发方面受到科学界的广泛关注，而 HA 涂层在氧化钛（TiO）纳米管上的应用显示出在提高细胞增殖、黏附和分化方面的潜在益处。本研究通过磁控溅射在 TiO 纳米管表面制备了 HA 涂层，以提高钛（Ti）表面的生物相容性。扫描电子显微镜（SEM）、表面轮廓仪和水接触角测量表明，HA 涂层的 TiO 纳米管影响了表面粗糙度（）和润湿性。XRD 和 FTIR 峰表明，在 500°C 下退火 120 分钟后，存在 TiO（锐钛矿）和 HA 涂层的 TiO 纳米管的结晶相。与原始阳极氧化的 TiO 纳米管和纯 CP-Ti 表面相比，HA 涂层的 TiO 纳米管具有明显更高的和更低的水接触角（θ）。使用成骨样细胞的 MTS 测定法证实，与原始阳极氧化的 TiO 纳米管和纯 CP-Ti 表面相比，HA 涂层的 TiO 纳米管表面提供了增强的细胞附着和生长。
