Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
Colloids Surf B Biointerfaces. 2020 Dec;196:111304. doi: 10.1016/j.colsurfb.2020.111304. Epub 2020 Aug 5.
Oxidative stress induced by reactive oxygen species (ROS) overproduction and accumulation would hinder the osseointegration process at the bone-implant interface, leading to a higher rate of implant failure. To endow titanium (Ti) implants with antioxidant activity, we developed a coating approach mediated by tannic acid (TA)-Ca coordination complexation. A hydroxyapatite (HA)/TA composite coating was prepared, based on Ti substrates modified by anodized and annealed titanium dioxide (TiO) nanotube arrays. The results reveal that highly ordered TiO nanotubes with a diameter of 142.23 ± 14.52 nm and a length of 374.17 ± 42.47 nm were fabricated on the Ti substrate and the XRD pattern shows the TiO anatase phase after annealing at 450 ℃. TA-Ca complexes were formed on the surface of TiO nanotubes by immersing the constructs into the mixed solution of TA and CaCl, where they are served as calcium sites for the HA growth by later phosphorylation. The HA nanoparticles present needle-shape with the diameter of 18 ∼ 20 nm. The total antioxidant capacity assay was employed to confirm the antioxidant effect of the HA/TA composite coating. The results indicate that it has a persistent and strong antioxidative activity. In vitro cytological test results show that HA/TA coating exhibits good cytocompatibility for osteoblasts proliferation and adhesion.
活性氧（ROS）过度产生和积累引起的氧化应激会阻碍骨-种植体界面的骨整合过程，导致更高的种植体失败率。为了赋予钛（Ti）植入物抗氧化活性，我们开发了一种通过单宁酸（TA）-Ca 配位络合介导的涂层方法。在经过阳极氧化和退火处理的二氧化钛（TiO）纳米管阵列修饰的 Ti 基底上制备了羟基磷灰石（HA）/TA 复合涂层。结果表明，在 Ti 基底上制备了直径为 142.23±14.52nm、长度为 374.17±42.47nm 的高度有序的 TiO 纳米管，并且 XRD 图谱显示在 450℃退火后存在 TiO 锐钛矿相。通过将构建体浸入 TA 和 CaCl 的混合溶液中，在 TiO 纳米管表面形成 TA-Ca 配合物，其中 TA-Ca 配合物充当 HA 通过后续磷酸化生长的钙源。HA 纳米颗粒呈针状，直径为 18∼20nm。总抗氧化能力测定法用于证实 HA/TA 复合涂层的抗氧化效果。结果表明，它具有持久而强大的抗氧化活性。体外细胞学试验结果表明，HA/TA 涂层对成骨细胞的增殖和黏附具有良好的细胞相容性。
