Biochemistry Section, Mansoura University Children's Hospital, Mansoura, Egypt.
Biochemistry Department, Faculty of Science, Damietta University, Damietta, Egypt.
Clin Biochem. 2021 Jul;93:66-72. doi: 10.1016/j.clinbiochem.2021.04.006. Epub 2021 Apr 20.
Interleukin 4 (IL4) is a key cytokine that regulates the inflammatory cascade in bronchial asthma. We investigated the association between the IL4 and IL4R polymorphisms and the susceptibility for bronchial asthma among Egyptian children.
IL4 VNTR and IL4R c.1902 A>G p.(Q576R) polymorphisms were investigated among 100 children with bronchial asthma and 100 healthy controls using PCR method. Serum levels of IL4 and immunoglobulin E (IgE) were assessed by ELISA.
The frequencies of (A1A2 + A2A2) genotypes and A2-allele of the IL4 VNTR variant were significantly higher among asthmatic patients than controls (p = 0.01, OR = 2.34, 95% CI = 1.24-4.44; p = 0.01, OR = 2.27, 95% CI = 1.29-3.99, respectively). The frequencies of (AG + GG) genotypes and G-allele of the IL4R (A1902G) variant were significantly higher among asthmatic patients than controls (p = 0.003, OR = 2.52, 95% CI = 1.39-4.58; p = 0.002, OR = 2.25, 95% CI = 1.35-3.76, respectively). There was a significant association between (A1A2 + A2A2) genotypes of the IL4 VNTR variant and high serum IL4 level among asthmatic patients (p < 0.001). The (AG + GG) genotypes of the IL4R (A1902G) variant were significantly associated with exposure to triggers, atopic dermatitis and higher serum IgE level in asthmatic patients (p = 0.02, 0.04 and 0.01, respectively).
IL4 VNTR and IL4R (A1902G) polymorphisms could be associated with higher risks of bronchial asthma among Egyptian children.
白细胞介素 4（IL4）是一种关键的细胞因子，可调节支气管哮喘中的炎症级联反应。我们研究了白细胞介素 4（IL4）和白细胞介素 4 受体（IL4R）多态性与埃及儿童支气管哮喘易感性之间的关系。
采用 PCR 法检测 100 例支气管哮喘患儿和 100 例健康对照者的白细胞介素 4 VNTR 和白细胞介素 4R c.1902A>G p.(Q576R)多态性。采用 ELISA 法检测血清白细胞介素 4 和免疫球蛋白 E（IgE）水平。
与对照组相比，哮喘患儿白细胞介素 4 VNTR 变异体的（A1A2+A2A2）基因型和 A2 等位基因的频率明显更高（p=0.01，OR=2.34，95%CI=1.24-4.44；p=0.01，OR=2.27，95%CI=1.29-3.99）。与对照组相比，哮喘患儿白细胞介素 4R（A1902G）变异体的（AG+GG）基因型和 G 等位基因的频率明显更高（p=0.003，OR=2.52，95%CI=1.39-4.58；p=0.002，OR=2.25，95%CI=1.35-3.76）。哮喘患儿白细胞介素 4 VNTR 变异体的（A1A2+A2A2）基因型与血清白细胞介素 4 水平升高显著相关（p<0.001）。白细胞介素 4R（A1902G）变异体的（AG+GG）基因型与哮喘患儿的触发因素暴露、特应性皮炎和血清 IgE 水平升高显著相关（p=0.02、0.04 和 0.01）。
白细胞介素 4 VNTR 和白细胞介素 4R（A1902G）多态性可能与埃及儿童支气管哮喘的较高风险相关。
