Sapartini Gartika, Wong Gary W K, Indrati Agnes Rengga, Kartasasmita Cissy B, Setiabudiawan Budi
Division of Allergy Immunology, Department of Child Health, Doctoral Study Program, Faculty of Medicine Universitas Padjadjaran, Bandung 40161, West Java, Indonesia.
Department of Paediatrics, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China.
Biomedicines. 2023 Sep 15;11(9):2542. doi: 10.3390/biomedicines11092542.
Children with stunted growth have an increased risk of wheezing, and studies have shown that low levels of vitamin D and interleukin (IL)-10, along with increased IL-4 levels and CD23+ expression, are present in stunted and asthmatic children. To date, it is not known whether these factors are related to the incidence of asthma in stunted children. This case-control study investigated the association between vitamin D, IL-4, and IL-10 levels and CD23+ expression with bronchial asthma in stunted children. The study included 99 children aged 24-59 months, i.e., 37 stunted-sthmatic children (cases), 38 stunted children without asthma, and 24 non-stunted children with asthma. All children were tested for their 25(OH)D levels using chemiluminescent immunoassay (CLIA), IL-4 and IL-10 levels were measured through enzyme-linked immunosorbent assay (ELISA) testing, and CD23+ expression was measured through flow cytometry bead testing. The data were analyzed using chi-squared, Kruskal-Wallis, and Mann-Whitney tests. The results showed that stunted asthmatic children had a higher incidence of atopic family members than those without asthma. Additionally, stunted asthmatic children had a higher prevalence of vitamin D deficiency (48.6%) than the control group (44.7% and 20.8%). Furthermore, stunted asthmatic children had significantly lower levels of 25(OH)D [20.55 (16.18-25.55), = 0.042] and higher levels of IL-4 [1.41 (0.95-2.40), = 0.038], although there were no significant differences in IL-10 levels and CD23+ expression. The study concluded that low vitamin D and high IL-4 levels are associated with bronchial asthma in stunted children, while IL-10 and CD23+ do not show a significant association.
生长发育迟缓的儿童患喘息的风险增加，研究表明，生长发育迟缓且患有哮喘的儿童体内维生素D和白细胞介素（IL）-10水平较低，同时IL-4水平升高和CD23+表达增加。迄今为止，尚不清楚这些因素是否与生长发育迟缓儿童的哮喘发病率有关。这项病例对照研究调查了生长发育迟缓儿童体内维生素D、IL-4和IL-10水平以及CD23+表达与支气管哮喘之间的关联。该研究纳入了99名年龄在24至59个月的儿童，即37名生长发育迟缓且患有哮喘的儿童（病例组）、38名生长发育迟缓但无哮喘的儿童以及24名非生长发育迟缓且患有哮喘的儿童。所有儿童均采用化学发光免疫分析法（CLIA）检测其25(OH)D水平，通过酶联免疫吸附测定法（ELISA）检测IL-4和IL-10水平，并通过流式细胞术磁珠检测法检测CD23+表达。数据采用卡方检验、Kruskal-Wallis检验和Mann-Whitney检验进行分析。结果显示，生长发育迟缓且患有哮喘的儿童中特应性家庭成员的发病率高于无哮喘的儿童。此外，生长发育迟缓且患有哮喘的儿童维生素D缺乏症的患病率（48.6%）高于对照组（44.7%和20.8%）。此外，生长发育迟缓且患有哮喘的儿童25(OH)D水平显著较低[20.55（16.18 - 25.55），P = 0.042]，IL-4水平较高[1.41（0.95 - 2.40），P = 0.038]，尽管IL-10水平和CD23+表达无显著差异。该研究得出结论，低维生素D水平和高IL-4水平与生长发育迟缓儿童的支气管哮喘有关，而IL-10和CD23+未显示出显著关联。
