Chemistry Department, Faculty of Science, Damietta University, Damietta, 34517, Egypt.
Genetics Unit, Children Hospital, Mansoura University, Mansoura, Egypt.
Sci Rep. 2022 Nov 7;12(1):18910. doi: 10.1038/s41598-022-21631-4.
Overproduction of mucins in the airways donates largely to airway blockage in asthma patients. Glycoprotein MUC7 plays a role in the clearance of bacteria and has anti-candidacidal criteria. Our goal was to investigate the association between the MUC7 variable number of tandem repeats (VNTR) polymorphism and bronchial asthma among Egyptian children. The MUC7 VNTR polymorphism was investigated among 100 children with bronchial asthma and 100 healthy controls using polymerase chain reaction (PCR) method. Serum levels of immunoglobulin E (IgE), tumor necrosis factor-alpha (TNF-α), and transforming growth factor-beta1 (TGF-β1) were assessed by enzyme-linked immunosorbent assay (ELISA) technique. The frequencies of 65 genotype, 55 genotype, (65 + 55) genotypes, and MUC75 allele of the MUC7 VNTR variant were significantly lower among asthmatic patients than controls (p < 0.015, OR = 0.39, 95% CI = 0.19-0.81; p = 0.03, OR = 0.18, 95% CI = 0.04-0.86; p < 0.001, OR = 0.29, 95% CI = 0.15-0.58; p < 0.001, OR = 0.3, 95% CI = 0.17-0.55, respectively). The (65 + 55) genotypes of the MUC7 VNTR variant were not associated with the clinical manifestations and serum levels of IgE, TNF-α, and TGF-β1 among asthmatic patients (p ˃ 0.05). In conclusion, the (65 + 5*5) genotypes of the MUC7 VNTR variant may have a protective role for bronchial asthma in Egyptian children.
气道中粘蛋白的过度产生在很大程度上导致了哮喘患者的气道阻塞。糖蛋白 MUC7 在清除细菌方面发挥作用，具有抗真菌作用。我们的目标是研究 MUC7 可变数串联重复（VNTR）多态性与埃及儿童支气管哮喘之间的关系。采用聚合酶链反应（PCR）方法检测 100 例支气管哮喘患儿和 100 例健康对照者的 MUC7 VNTR 多态性。采用酶联免疫吸附试验（ELISA）技术检测血清免疫球蛋白 E（IgE）、肿瘤坏死因子-α（TNF-α）和转化生长因子-β1（TGF-β1）水平。MUC7 VNTR 变异体的 65 基因型、55 基因型、（65+55）基因型和 MUC75 等位基因的频率在哮喘患者中明显低于对照组（p<0.015，OR=0.39，95%CI=0.19-0.81；p=0.03，OR=0.18，95%CI=0.04-0.86；p<0.001，OR=0.29，95%CI=0.15-0.58；p<0.001，OR=0.3，95%CI=0.17-0.55）。MUC7 VNTR 变异体的（6*5+5*5）基因型与哮喘患者的临床表现及血清 IgE、TNF-α和 TGF-β1 水平无关（p>0.05）。结论：MUC7 VNTR 变异体的（65+5*5）基因型可能对埃及儿童支气管哮喘具有保护作用。
