The United Graduate School of Agricultural Sciences, Iwate University, Morioka, 020-8550, Japan.
Graduate School of Agriculture and Life Science, Hirosaki University, Hirosaki, 036-8561, Japan.
Biochem Biophys Res Commun. 2021 Jun 11;557:104-109. doi: 10.1016/j.bbrc.2021.03.143. Epub 2021 Apr 14.
Cel7 RNA is a member of the Caenorhabditis elegans stem-bulge RNAs (sbRNAs) that are classified into the Y RNA family based on their structural similarity. We identified a 15-nucleotide-shorter form of Cel7 RNA and designated it Cel7s RNA. Both Cel7 and Cel7s RNAs increased during the development of worms from L1 to adult. Cel7s RNA was notably more abundant in embryos than in L1 to L3 larvae. Cel7 RNA in embryo was less than those in L2 to adult. The ratio of cellular level of Cel7 RNA to that of Cel7s RNA was higher in L1 to L4, but reversed in embryos and adults. In rop-1 mutants, in which the gene for the C. elegans Ro60 homolog, ROP-1, was disrupted, Cel7s RNA decreased similar to CeY RNA, another C. elegans Y RNA homolog. Surprisingly, Cel7 RNA, existed stably in the absence of ROP-1, unlike Cel7s and CeY RNAs. Gel-shift assays demonstrated that Cel7 and Cel7s RNAs bound to ROP-1 in a similar manner, which was much weaker than CeY RNA. The 5'-terminal 15-nt of Cel7 RNA could be folded into a short stem-loop structure, probably contributing to the stability of Cel7 RNA in vivo and the distinct expression patterns of the 2 RNAs.
Cel7 RNA 是秀丽隐杆线虫茎膨突 RNA（sbRNAs）的成员之一，根据其结构相似性被归类为 Y RNA 家族。我们鉴定出 Cel7 RNA 的一种 15 个核苷酸缩短形式，并将其命名为 Cel7s RNA。Cel7 和 Cel7s RNA 在从 L1 到成虫的线虫发育过程中均增加。Cel7s RNA 在胚胎中的丰度明显高于 L1 到 L3 幼虫。Cel7 RNA 在胚胎中的丰度则低于 L2 到成虫。Cel7 RNA 与 Cel7s RNA 的细胞水平比值在 L1 到 L4 较高，但在胚胎和成虫中则相反。在 rop-1 突变体中，破坏了秀丽隐杆线虫 Ro60 同源物 ROP-1 的基因，Cel7s RNA 下降类似于 CeY RNA，另一种秀丽隐杆线虫 Y RNA 同源物。令人惊讶的是，Cel7 RNA 在没有 ROP-1 的情况下稳定存在，而 Cel7s 和 CeY RNA 则不然。凝胶迁移分析表明，Cel7 和 Cel7s RNA 以相似的方式与 ROP-1 结合，其结合能力比 CeY RNA 弱得多。Cel7 RNA 的 5'-末端 15 个核苷酸可以折叠成一个短的茎环结构，可能有助于 Cel7 RNA 在体内的稳定性以及这 2 种 RNA 的独特表达模式。
