Liu Cai, Yu Yanchong, Liu Hanzhu, Xin Hua
University Key Laboratory of Plant Biotechnology in Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
Plant Physiol Biochem. 2021 Jun;163:205-214. doi: 10.1016/j.plaphy.2021.03.051. Epub 2021 Apr 5.
In this study, soybean (Glycine max) seeds were cultured in distilled water. When the roots were about 2 cm, they were separately treated with copper oxide bulk particles (CuO BPs) suspensions and copper oxide nanoparticle (CuO NPs) suspensions in different concentrations (2, 5 and 10 mg L) for 24 h and 48 h. Results showed that different concentrations of CuO BPs suspensions had little effect on the structure and cell division of meristematic zone. After CuO NPs treatment, Cu content increased in the roots, accompanied by high reactive oxygen species, malondialdehyde and relative electrical conductivity. CuO NPs significantly inhibited the growth of soybean roots over exposure time and the concentration. The destruction of CuO NPs occurred first in the promeristem, and then in the primary meristem of the meristematic zone. The meristematic cells of roots showed vacuolization, the nuclei swelled and deformed. After 10 mg L CuO NPs treatment for 48 h, the mitotic index of root cells decreased by 14.28%, and the micronucleus rate increased by 14.33‰. Some cell division genes, such as GmCYCB1; 2, GmCYCU4; 1, GmCYCA1; 1, GmCYCP3; 1, GmCYCD3; 1 and CDC20; 1 were up-regulated or down-regulated with CuO NPs treatments.
在本研究中，大豆（Glycine max）种子在蒸馏水中培养。当根长到约2厘米时，将其分别用不同浓度（2、5和10毫克/升）的氧化铜块状颗粒（CuO BPs）悬浮液和氧化铜纳米颗粒（CuO NPs）悬浮液处理24小时和48小时。结果表明，不同浓度的CuO BPs悬浮液对分生区的结构和细胞分裂影响较小。经CuO NPs处理后，根中铜含量增加，同时活性氧、丙二醛和相对电导率升高。CuO NPs在暴露时间和浓度上均显著抑制大豆根的生长。CuO NPs的破坏首先发生在原分生组织，然后发生在分生区的初生分生组织。根的分生细胞出现液泡化，细胞核肿胀变形。经10毫克/升CuO NPs处理48小时后，根细胞的有丝分裂指数下降了14.28%，微核率增加了14.33‰。一些细胞分裂基因，如GmCYCB1;2、GmCYCU4;1、GmCYCA1;1、GmCYCP3;1、GmCYCD3;1和CDC20;1在CuO NPs处理后上调或下调。
