Brandes Bettina, Godbold Douglas L, Kuhn Arnd J, Jentschke Georg
Forest Ecosystem Research Centre, Institute of Forest Botany, University of Göttingen, Büsgenweg 2, D-37077 Göttingen, Germany.
School of Agricultural and Forest Sciences, University of Wales Bangor, Bangor, Gwynedd LL57 2UW, UK.
New Phytol. 1998 Dec;140(4):735-743. doi: 10.1046/j.1469-8137.1998.00313.x.
The contribution of the extramatrical mycelium to N and P nutrition of mycorrhizal Norway spruce (Picea abies (L.) Karst.) was investigated. Seedlings either inoculated with Paxillus involutus (Batsch) Fr. or non-mycorrhizal were grown in a two compartment sand culture system where hyphae were separated from roots by a 45 μm nylon net. Nutrient solution of the hyphal compartment contained either 1.8 mm NH and 0.18 mm H PO or no N and P. Aluminium added to the hyphal compartment as a tracer of mass flow was not detected in the plant compartment, indicating that measurements of N and P transfer by the mycelium were not biased by solute movement across the nylon net. The addition of N and P to the hyphal compartment markedly increased dry weight, N and P concentration and N and P content of mycorrhizal plants. Calculating uptake from the difference in input and output of nutrient in solution confirmed a hyphal contribution of 73% and 76% to total N and P uptake, respectively. Hyphal growth was increased at the site of nutrient solution input.
研究了菌根外菌丝体对菌根化挪威云杉（Picea abies (L.) Karst.）氮和磷营养的贡献。接种卷缘桩菇（Paxillus involutus (Batsch) Fr.）或未形成菌根的幼苗在两室砂培系统中生长，其中菌丝体通过45μm尼龙网与根系分离。菌丝体隔室的营养液含有1.8 mM NH₄⁺和0.18 mM H₂PO₄⁻或不含氮和磷。作为质量流示踪剂添加到菌丝体隔室的铝在植物隔室中未被检测到，这表明菌丝体对氮和磷转移的测量不受溶质穿过尼龙网移动的影响。向菌丝体隔室添加氮和磷显著增加了菌根化植物的干重、氮和磷浓度以及氮和磷含量。通过计算溶液中养分输入和输出的差异来确定吸收量，结果证实菌丝体对总氮和磷吸收的贡献分别为73%和76%。在营养液输入部位，菌丝体生长增加。
