Hodson M J, Wilkins D A
School of Biological Sciences, University of Birmingham, Birmingham B15 2TT, UK.
New Phytol. 1991 Jun;118(2):273-278. doi: 10.1111/j.1469-8137.1991.tb00977.x.
Norway spruce [Picea abies (L.) Karst.] seed lots from populations growing on acid soil in the Black Forest (BF), and from a calcareous soil in the Schwäbische Alb (SA), West Germany, were grown in perlite and treated with 0 and 6 mM Al. Some of the plants were inoculated with the fungus Paxillus involutes Fr., while some were not. Fungus was associated with the roots of the inoculated plants, but mycorrhizas did not form. Mineral element distribution in the roots was investigated using X-ray microanalysis of freeze substituted sections in TEM. Seven elements were detected: aluminium, silicon, phosphorus, sulphur, chlorine, potassium, and calcium. Aluminium was almost entirely confined to the cortical cell walls, and was not detectable inside the endodermis. The presence of P. involutus significantly increased aluminium concentrations in the cortical cell walls of both seed lots, while silicon concentrations in the aluminium-tolerant (BF) plants increased in response to aluminium treatment.
来自德国西部黑森林（BF）酸性土壤上生长的挪威云杉[Picea abies (L.) Karst.]种群以及施瓦本汝拉山（SA）钙质土壤上的种子批次，种植在珍珠岩中，并用0和6 mM的铝进行处理。部分植株接种了真菌卷缘桩菇（Paxillus involutes Fr.），部分未接种。真菌与接种植株的根系相关联，但未形成菌根。通过透射电子显微镜（TEM）对冷冻置换切片进行X射线微分析，研究了根系中矿质元素的分布。检测到七种元素：铝、硅、磷、硫、氯、钾和钙。铝几乎完全局限于皮层细胞壁，在内皮层内部无法检测到。卷缘桩菇的存在显著增加了两个种子批次皮层细胞壁中的铝浓度，而耐铝（BF）植株中的硅浓度则因铝处理而增加。
