Ulrich Kristina, Lentzsch Peter, Seyfarth Wolfgang
Centre for Agricultural Landscape and Land Use Research Müncheberg, Institute of Microbial Ecology and Soil Biology, Eberswalder Str. 84, D-15374 Müncheberg, Germany.
New Phytol. 1997 Oct;137(2):285-291. doi: 10.1046/j.1469-8137.1997.00797.x.
The components of leghaemoglobin (Lb) from twelve different Pisum sativum L. cvs and three near-isogenic foliar mutants were investigated by anion-exchange high-performance liquid chromatography (HPLC). Five different Lb component profiles could he found. The number of components varied from four to six dependent on cultivar used. An Lb pattern composed of four Lb components could be detected in thirteen P. sativum cultivars and lines. Ten of them showed an identical profile. In nodules of each cultivar, the two known major components, LbI and LbV, but also LbIV, could be detected. Additionally, cultivar-specific Lb components could be identified, each representing up to 10%, of total Lb. One of these components, LbIII, has been described previously, but three new Lb components (LbII, LbVI, and LbVII) were found. The presence of all Lb components detected by HPLC was confirmed by analytical isoelectric focusing. Further, it was shown that age-dependent changes in the relative concentrations of LbI and LbV are common in P. sativum and that these variations are independent of breeding lines and cultivars.
采用阴离子交换高效液相色谱法（HPLC）对12个不同的豌豆（Pisum sativum L.）品种和3个近等基因叶突变体的豆血红蛋白（Lb）成分进行了研究。发现了5种不同的Lb成分谱。成分数量因所用品种而异，从4种到6种不等。在13个豌豆品种和品系中检测到由4种Lb成分组成的Lb模式。其中10个显示出相同的谱图。在每个品种的根瘤中，可检测到两种已知的主要成分LbI和LbV，以及LbIV。此外，还可鉴定出品种特异性的Lb成分，每种成分占总Lb的比例高达10%。其中一种成分LbIII先前已有描述，但发现了3种新的Lb成分（LbII、LbVI和LbVII）。通过分析等电聚焦证实了HPLC检测到的所有Lb成分的存在。此外，研究表明，LbI和LbV相对浓度随年龄的变化在豌豆中很常见，且这些变化与育种品系和品种无关。
