Bogusz D, Kortt A A, Appleby C A
Arch Biochem Biophys. 1987 Apr;254(1):263-71. doi: 10.1016/0003-9861(87)90102-0.
By anion-exchange chromatography, the nitrogen fixing photosynthetic stem nodules and nonphotosynthetic root nodules of Sesbania rostrata are shown to contain the same seven major components of leghemoglobin (Lb), numbered LbI-LbVII in order of elution, although in different proportions. No novel component was found in photosynthetic nodules. All components of Sesbania Lb are monomeric, with molecular weights varying between 15,000 and 17,000, and at least six of them are separate gene products. It is suspected that variable conjugation with nonprotein moieties might be partially responsible for the molecular weight differences and anomalous behavior observed between isoelectric focusing and anion-exchange chromatography.
通过阴离子交换色谱法显示，罗望子的固氮光合茎瘤和非光合根瘤含有相同的七种主要豆血红蛋白（Lb）成分，按照洗脱顺序编号为LbI - LbVII，尽管比例不同。在光合结节中未发现新成分。罗望子Lb的所有成分都是单体，分子量在15,000至17,000之间变化，其中至少六种是不同的基因产物。据推测，与非蛋白质部分的可变结合可能部分导致了在等电聚焦和阴离子交换色谱法之间观察到的分子量差异和异常行为。
