McKee I F, Eiblmeier M, Polle A
Department of Biological Sciences, John Tabor Laboratories, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ, UK.
Institut für Forstbotanik und Baumphysiologie, Albert-Ludwigs-Universität Freiburg, Am Flughafen 17, D-79085, Freiburg, Germany.
New Phytol. 1997 Oct;137(2):275-284. doi: 10.1046/j.1469-8137.1997.00801.x.
Elevated [CO ] has been shown to protect photosynthesis and growth of wheat against moderately elevated [O ]. To investigate the role of ozone exclusion and detoxification in this protection, spring wheat (Triticum aestivum L. ev. Wembley) was grown from seed, in controlled-environment chambers, under reciprocal combinations of [CO ] at 350 or 700 μmol mol and [O ] peaking at < 5 or 60 nmol mol , respectively. Cumulative ozone dose to the mesophyll and antioxidant status were determined throughout flag leaf development. Catalase activity correlated with rates of photorespiration and declined in response to elevated [CO ] and/or [O ]. Superoxide dismutase activity was not significantly affected by either condition. Neither ascorbate nor glutathione content was enhanced by elevated [CO ]. In wheat, at moderately elevated [O ], our results show that stomatal exclusion plays a major role in the protective effect of elevated [CO ] against O damage.
已表明，升高的[CO₂]可保护小麦的光合作用和生长，使其免受适度升高的[O₃]的影响。为了研究臭氧排除和解毒在这种保护作用中的作用，将春小麦（Triticum aestivum L. ev. Wembley）从种子开始，种植在可控环境室内，[CO₂]分别为350或700 μmol mol⁻¹，[O₃]峰值分别为<5或60 nmol mol⁻¹的相互组合条件下。在整个旗叶发育过程中，测定了叶肉的累积臭氧剂量和抗氧化状态。过氧化氢酶活性与光呼吸速率相关，并随着[CO₂]和/或[O₃]升高而下降。超氧化物歧化酶活性不受这两种条件的显著影响。升高[CO₂]既没有提高抗坏血酸含量，也没有提高谷胱甘肽含量。在小麦中，在适度升高[O₃]的情况下，我们的结果表明，气孔排除在升高的[CO₂]对O₃损害的保护作用中起主要作用。
