Sun Chuanxin, Sathish P, Ahlandsberg Staffan, Deiber Anna, Jansson Christer
Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, S-10691 Stockholm, Sweden.
New Phytol. 1997 Oct;137(2):215-222. doi: 10.1046/j.1469-8137.1997.00796.x.
Developing caryopses of barley (Hardeum vulgare L. ev. Golf) were monitored for starch-branching enzyme activity for 1 month after anthesis. Homogenized caryopses from grains with the highest specific activity were used as starting material for purification of branching enzyme by FPLC chromatography. Several branching enzyme activity fractions were resolved. We have previously described the isolation of a 51/50 kDa protein with starch branching activity from one of these fractions. From three other fractions, we have now partially purified starch branching enzymes corresponding to forms I, IIa and IIb.
在大麦（Hordeum vulgare L. ev. Golf）开花后1个月内，监测其正在发育的颖果中的淀粉分支酶活性。具有最高比活性的谷粒中的匀浆颖果用作通过快速蛋白质液相色谱（FPLC）纯化分支酶的起始材料。分离出了几个具有分支酶活性的组分。我们之前已经描述过从其中一个组分中分离出一种具有淀粉分支活性的51/50 kDa蛋白质。现在，我们从其他三个组分中部分纯化了与I型、IIa型和IIb型相对应的淀粉分支酶。
