Yang Ruifang, Bai Jianjiang, Fang Jun, Wang Ying, Lee Gangseob, Piao Zhongze
Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences , 1000 Jingqi Road, Fengxian District, Shanghai 201403 , China.
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences , 1000 Jingqi Road, Fengxian District, Shanghai 201403 , China.
Breed Sci. 2016 Sep;66(4):481-489. doi: 10.1270/jsbbs.16037. Epub 2016 Jul 13.
Foods rich in resistant starch can help prevent various diseases, including diabetes, colon cancers, diarrhea, and chronic renal and hepatic diseases. Variations in starch biosynthesis enzymes could contribute to the high content of resistant starch in some cultivars of rice ( L.). Our previously published work indicated that the gene in the rice mutant line, 'Jiangtangdao1' was a putative allele of the rice starch branching enzyme gene (previously known as ); might control the biosynthesis of the high resistant starch content in the rice line. Biomolecular analysis showed that the activity of SBEs was significantly lower in soluble extracts of immature seeds harvested from 'Jiangtangdao1' 15 days after flowering than in the extracts of the wild-type rice line 'Huaqingdao'. We performed gene complementation assays by introducing the wild-type into the mutant 'Jiangtangdao1'. The genetically complemented lines demonstrated restored seed-related traits. The structures of endosperm amylopectin and the morphological and physicochemical properties of the starch granules in the transformants recovered to wild-type levels. This study provides evidence that is a novel allele of , responsible for biosynthesis of high resistant starch in 'Jiangtangdao1'.
富含抗性淀粉的食物有助于预防多种疾病，包括糖尿病、结肠癌、腹泻以及慢性肾脏和肝脏疾病。淀粉生物合成酶的变异可能导致某些水稻品种中抗性淀粉含量较高。我们之前发表的研究表明，水稻突变系“江糖稻1号”中的基因是水稻淀粉分支酶基因（之前称为）的一个假定等位基因；可能控制该水稻品系中高抗性淀粉含量的生物合成。生物分子分析表明，开花后15天从“江糖稻1号”收获的未成熟种子的可溶性提取物中，淀粉分支酶（SBEs）的活性显著低于野生型水稻品系“华清稻”的提取物。我们通过将野生型导入突变体“江糖稻1号”进行了基因互补试验。基因互补系表现出恢复的种子相关性状。胚乳支链淀粉的结构以及转化体中淀粉颗粒的形态和物理化学性质恢复到了野生型水平。本研究提供了证据，表明是一个新的等位基因，负责“江糖稻1号”中高抗性淀粉的生物合成。
