Bean Gregory J, Marks M David, Hülskamp Martin, Clayton Murray, Croxdale Judith L
Department of Botany, University of Wisconsin, Madison, WI 53706, USA.
Department of Plant Biology, University of Minnesota, St. Paul, MN 55108, USA.
New Phytol. 2002 Mar;153(3):461-467. doi: 10.1046/j.0028-646X.2001.00342.x. Epub 2002 Mar 5.
• Trichome and stomatal patterning are not independent events because trichomes form before stomata. We thought trichome genes might provide spatial referents to ensure proper distribution of stomata for gas exchange, and therefore studied mutants of GL1 and TRY using stomatal pattern of the entire cotyledon surface as the indicator. • Mature cotyledons were imaged by SEM, stomatal maps were generated, and data were spatially analysed. Expression of GL1 and TRY was determined in wild type and mutant samples by reverse transcriptase-PCR (RT-PCR) analysis. • At the tissue level, findings showed wild type cotyledons had a random stomatal pattern, whereas gl1-1 and try240 cotyledons had ordered and clustered stomatal patterns, respectively. Regardless of overall pattern type, c. 10% of the stomatal population - those closest to one another - were always ordered, the result of genes regulating cellular differentiation. • These results indicate epidermal cells respond to GL1 and TRY signals that affect distribution of both stomata and trichomes in postembryogenic events. The GL1 and TRY genes play dual roles in the epidermis, one role regulating epidermal tissue patterning and a second role connected with trichome development.
• 表皮毛和气孔模式并非独立事件，因为表皮毛在气孔之前形成。我们认为表皮毛基因可能提供空间参考，以确保气孔为气体交换进行适当分布，因此以整个子叶表面的气孔模式为指标，研究了GL1和TRY的突变体。
• 用扫描电子显微镜对成熟子叶成像，生成气孔图谱，并进行空间数据分析。通过逆转录聚合酶链反应（RT-PCR）分析确定野生型和突变体样本中GL1和TRY的表达。
• 在组织水平上，研究结果表明野生型子叶具有随机的气孔模式，而gl1-1和try240子叶分别具有有序和聚集的气孔模式。无论总体模式类型如何，约10%的气孔群体（彼此最接近的那些）总是有序的，这是调节细胞分化的基因的结果。
• 这些结果表明，表皮细胞对GL1和TRY信号作出反应，这些信号在胚胎后事件中影响气孔和表皮毛的分布。GL1和TRY基因在表皮中发挥双重作用，一个作用是调节表皮组织模式，另一个作用与表皮毛发育有关。
