与亚洲谱系寨卡病毒相关的亚基因组黄病毒 RNA（sfRNA）在乌干达三种蝙蝠（翼手目蝙蝠科）中被发现。
Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae).
机构信息
Colorado State University, Fort Collins, CO, USA.
Centers for Disease Control and Prevention, Fort Collins, CO, USA.
出版信息
Sci Rep. 2021 Apr 16;11(1):8370. doi: 10.1038/s41598-021-87816-5.
Serological cross-reactivity among flaviviruses makes determining the prior arbovirus exposure of animals challenging in areas where multiple flavivirus strains are circulating. We hypothesized that prior infection with ZIKV could be confirmed through the presence of subgenomic flavivirus RNA (sfRNA) of the 3' untranslated region (UTR), which persists in tissues due to XRN-1 stalling during RNA decay. We amplified ZIKV sfRNA but not NS5 from three experimentally-infected Jamaican fruit bats, supporting the hypothesis of sfRNA tissue persistence. Applying this approach to 198 field samples from Uganda, we confirmed presence of ZIKV sfRNA, but not NS5, in four bats representing three species: Eidolon helvum (n = 2), Epomophorus labiatus (n = 1), and Rousettus aegyptiacus (n = 1). Amplified sequence was most closely related to Asian lineage ZIKV. Our results support the use of sfRNA as a means of identifying previous flavivirus infection and describe the first detection of ZIKV RNA in East African bats.
黄病毒之间的血清交叉反应使得在多种黄病毒株流行的地区确定动物先前的虫媒病毒暴露情况具有挑战性。我们假设，通过存在 3'非翻译区（UTR）的亚基因组黄病毒 RNA（sfRNA）可以确认先前感染寨卡病毒，因为在 RNA 衰变期间 XRN-1 停滞，sfRNA 会在组织中持续存在。我们从三名感染寨卡病毒的牙买加果蝠中扩增了 ZIKV sfRNA，但未从 NS5 中扩增出来，这支持了 sfRNA 组织持久性的假设。将该方法应用于来自乌干达的 198 个现场样本，我们在四只代表三个物种的蝙蝠中确认存在 ZIKV sfRNA（n=2），但未发现 NS5（n=1），分别是 Eidolon helvum、Epomophorus labiatus 和 Rousettus aegyptiacus。扩增的序列与亚洲谱系 ZIKV 最为密切相关。我们的研究结果支持将 sfRNA 作为识别先前的黄病毒感染的一种手段，并描述了首次在东非蝙蝠中检测到寨卡病毒 RNA。
Zotero 插件