1Arbovirology Department, Uganda Virus Research Institute, Entebbe, Uganda.
2Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA.
J Gen Virol. 2018 Sep;99(9):1248-1252. doi: 10.1099/jgv.0.001113. Epub 2018 Jul 5.
Zika virus (ZIKV), transmitted by Aedes species mosquitoes, was first isolated in Uganda in 1947. From February 2014 to October 2017, the Uganda Virus Research Institute, in collaboration with the US Centers for Diseases Control and Prevention, conducted arbovirus surveillance in acute febrile illness (AFI) patients at St Francis hospital in Nkonkonjeru. Three hundred and eighty-four serum samples were collected and tested for IgM antibodies to yellow fever virus (YFV), West Nile virus (WNV), dengue virus (DENV), chikungunya virus (CHIKV) and ZIKV. Of the 384 samples, 5 were positive for ZIKV IgM. Of these five, three were confirmed by plaque reduction neutralization test (PRNT) to be ZIKV infections. Of the remaining two, one was determined to be a non-specific flavivirus infection and one was confirmed to be alphavirus-positive by reverse transcriptase polymerase chain reaction (RT-PCR). This study provides the first evidence of laboratory-confirmed ZIKV infection in Uganda in five decades, and emphasizes the need to enhance sentinel surveillance.
寨卡病毒（ZIKV）由伊蚊属蚊子传播，于 1947 年首次在乌干达分离得到。2014 年 2 月至 2017 年 10 月，乌干达病毒研究所与美国疾病控制与预防中心合作，在 Nkonkonjeru 的圣弗朗西斯医院对急性发热性疾病（AFI）患者进行虫媒病毒监测。共采集了 384 份血清样本，用于检测黄热病病毒（YFV）、西尼罗河病毒（WNV）、登革热病毒（DENV）、基孔肯雅病毒（CHIKV）和寨卡病毒（ZIKV）的 IgM 抗体。在 384 份样本中，有 5 份寨卡病毒 IgM 呈阳性。其中 3 份经蚀斑减少中和试验（PRNT）确认为寨卡病毒感染。剩余的 2 份中，1 份为非特异性黄病毒感染，1 份经逆转录聚合酶链反应（RT-PCR）确认为正粘病毒阳性。本研究首次在乌干达提供了近 50 年来实验室确诊的寨卡病毒感染证据，强调需要加强哨点监测。
