School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima, Peru; Facultad de Ciencias de la Salud, Universidad Tecnológica del Peru, Lima, Peru.
Facultad de Ciencias, Universidad Nacional de San Antonio Abad del Cusco (UNSAAC), Cusco, Peru; Instituto Universitario de Enfermedades Tropicales y Biomedicina del Cusco-UNSAAC, Cusco, Peru.
J Infect Public Health. 2021 May;14(5):670-673. doi: 10.1016/j.jiph.2021.02.005. Epub 2021 Feb 23.
To date, there have been no molecular typing studies to identify the Sporothrix species circulating in Abancay, a hyperendemic area of sporotrichosis in Peru. To identify six clinical isolates of the Sporothrix schenckii complex from Abancay, Peru, we used PCR-sequencing of the calmodulin gene, and a phylogenetic analysis was conducted with these and additional sequences from GenBank. All clinical isolates were identified as S. schenckii (sensu stricto). Phylogenetic analysis revealed that the six clinical isolates from Abancay, Peru clustered in a clade along with sequences from Costa Rica, Iran, South Africa, and four other sequences from Peru. These findings reveal the presence of S. schenckii (sensu stricto) in Abancay, Peru.
迄今为止,尚无分子分型研究来确定秘鲁阿班凯(sporotrichosis 的高度流行地区)流行的孢子丝菌属菌种。为了鉴定来自秘鲁阿班凯的 6 株申克孢子丝菌复合体临床分离株,我们使用钙调蛋白基因的 PCR 测序,并对这些序列以及来自 GenBank 的其他序列进行了系统发育分析。所有临床分离株均被鉴定为申克孢子丝菌(严格意义上)。系统发育分析显示,来自秘鲁阿班凯的 6 株临床分离株与来自哥斯达黎加、伊朗、南非和秘鲁的另外 4 个序列聚集在一个分支中。这些发现表明,秘鲁阿班凯存在严格意义上的申克孢子丝菌。