诱导多能干细胞系（CSUi004-A），来源于健康个体的皮肤成纤维细胞。
An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual.
机构信息
Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China.
Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, China; Provincial Clinical Research Center for Rheumatic and Immunologic Diseases, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China.
出版信息
Stem Cell Res. 2021 May;53:102336. doi: 10.1016/j.scr.2021.102336. Epub 2021 Apr 8.
We established an induced pluripotent stem cell (iPSC) reprogrammed from dermal fibroblasts of a 53-year-old healthy man. Retroviruses expressing Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) were employed to initiate the reprogramming and were silenced in iPSCs. The generated iPSCs were pluripotent as determined by immunostaining and expression of pluripotency markers. They were further induced as embryoid bodies in vitro and teratomas in vivo, reminiscent of their full capacity of differentiating into three germ layers. They are with a normal karyotype and genetically identical to donor fibroblasts. This iPSC line provides excellent cell sources for studying and modeling human-specific physiology.
我们从一位 53 岁健康男性的皮肤成纤维细胞中建立了一个诱导多能干细胞（iPSC）。我们使用表达山中因子（OCT4、SOX2、KLF4 和 c-MYC）的逆转录病毒来启动重编程，并在 iPSC 中沉默这些基因。通过免疫染色和多能性标记物的表达，证明生成的 iPSC 具有多能性。进一步将它们在体外诱导为类胚体，并在体内诱导为畸胎瘤，这提示它们具有向三个胚层分化的全部潜能。它们具有正常的核型，并且在遗传上与供体成纤维细胞完全相同。这条 iPSC 系为研究和模拟人类特有的生理机能提供了极好的细胞来源。
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