Department of Radiology, Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School), No. 568, North Zhongxing Road, Yuecheng District, Shaoxing City, 312000, Zhejiang Province, China.
Department of Pathology, Shaoxing People's Hospital (Shaoxing Hospital, Zhejiang University School), Shaoxing, 312000, China.
BMC Med Imaging. 2021 Apr 17;21(1):73. doi: 10.1186/s12880-021-00604-5.
To explore if the quantitative perfusion histogram parameters of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) correlates with the expression of PTEN, P-Akt and m-TOR protein in lung cancer.
Thirty-three patients with 33 lesions who had been diagnosed with lung cancer were enrolled in this study. They were divided into three groups: squamous cell carcinoma (SCC, 15 cases), adenocarcinoma (AC, 12 cases) and small cell lung cancer (SCLC, 6 cases). Preoperative imaging (conventional imaging and DCE-MRI) was performed on all patients. The Exchange model was used to measure the phar- macokinetic parameters, including K, V, K, V and F, and then the histogram parameters meanvalue, skewness, kurtosis, uniformity, energy, entropy, quantile of above five parameters were analyzed. The expression of PTEN, P-Akt and m-TOR were assessed by immunohistochemistry. Spearman correlation analysis was used to compare the correlation between the quantitative perfusion histogram parameters and the expression of PTEN, P-Akt and m-TOR in different pathological subtypes of lung cancer.
The expression of m-TOR (P = 0.013) and P-Akt (P = 0.002) in AC was significantly higher than those in SCC. V (uniformity) in SCC group, K (uniformity), V (kurtosis, Q10, Q25) in AC group, F (skewness, kurtosis, energy), V (Q75, Q90, Q95) in SCLC group was positively correlated with PTEN, and F (entropy) in the SCLC group was negatively correlated with PTEN (P < 0.05); K (Q5, Q10) in the SCLC group was positively correlated with P-Akt, and K (energy) in the SCLC group was negatively correlated with P-Akt (P < 0.05); K (Q5) in SCC group and V (meanvalue, Q75, Q90, Q95) in SCLC group was positively correlated with m-TOR, and V (meanvalue) in SCC group was negatively correlated with m-TOR (P < 0.05).
The quantitative perfusion histogram parameters of DCE-MRI was correlated with the expression of PTEN, P-Akt and m-TOR in different pathological types of lung cancer, which may be used to indirectly evaluate the activation status of PI3K/Akt/mTOR signal pathway gene in lung cancer, and provide important reference for clinical treatment.
探讨动态对比增强磁共振成像（DCE-MRI）的定量灌注直方图参数是否与肺癌中 PTEN、P-Akt 和 m-TOR 蛋白的表达相关。
本研究纳入了 33 名经诊断患有肺癌的患者，共 33 处病灶。他们被分为三组：鳞状细胞癌（SCC，15 例）、腺癌（AC，12 例）和小细胞肺癌（SCLC，6 例）。所有患者均接受了术前影像学（常规影像学和 DCE-MRI）检查。使用交换模型测量药代动力学参数，包括 K、V、K、V 和 F，然后分析直方图参数均值、偏度、峰度、均匀性、能量、熵和五个参数的分位数。通过免疫组织化学评估 PTEN、P-Akt 和 m-TOR 的表达。采用 Spearman 相关性分析比较不同肺癌病理亚型中定量灌注直方图参数与 PTEN、P-Akt 和 m-TOR 表达之间的相关性。
AC 中 m-TOR（P=0.013）和 P-Akt（P=0.002）的表达明显高于 SCC。SCC 组中 V（均匀性）、AC 组中 K（均匀性）、V（峰度、Q10、Q25）、SCLC 组中 F（偏度、峰度、能量）、V（Q75、Q90、Q95）与 PTEN 呈正相关，SCLC 组中 F（熵）与 PTEN 呈负相关（P<0.05）；SCLC 组中 K（Q5、Q10）与 P-Akt 呈正相关，SCLC 组中 K（能量）与 P-Akt 呈负相关（P<0.05）；SCC 组中 K（Q5）和 SCLC 组中 V（平均值、Q75、Q90、Q95）与 m-TOR 呈正相关，SCC 组中 V（平均值）与 m-TOR 呈负相关（P<0.05）。
DCE-MRI 的定量灌注直方图参数与不同病理类型肺癌中 PTEN、P-Akt 和 m-TOR 的表达相关，这可能有助于间接评估肺癌中 PI3K/Akt/mTOR 信号通路基因的激活状态，为临床治疗提供重要参考。
