Department of Health Care, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, 266035, China.
Department of Geratology, Jinan Zhangqiu District Hospital of TCM, Jinan, 250200, China.
World J Surg Oncol. 2021 Apr 17;19(1):121. doi: 10.1186/s12957-021-02176-8.
It has been reported that long-chain non-coding RNA (lncRNA) zinc finger E-box binding homeobox 1 antisense 1 (ZEB1-AS1) is an oncogene in various cancers, including hepatocellular carcinoma (HCC). We investigated the role and mechanism of ZEB1-AS1 as a competitive endogenous RNA (ceRNA) combined with miR-23c in HCC cell proliferation and invasion.
QRT-PCR was used to detect ZEB1-AS1 and miR-23c expressions in HCC tissues and cells. The dual luciferase reporter assay detected the targeted regulation of miR-23c and ZEB1-AS1. We also performed the correlation analysis of their expression in HCC tissues by the Spearman's correlation analysis. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to detect the proliferation of hepatoma cells. Cell invasion was assessed by the Transwell assay.
QRT-PCR results indicated ZEB1-AS1 was upregulated and miR-23c was downregulated in HCC tissues and cell lines. ZEB1-AS1 knockdown hampered the proliferation and invasion of HCC cells. Dual luciferase reporter assay showed that miR-23c is a target of ZEB1-AS1, and ZEB1-AS1 was significantly negatively correlated with the miR-23c expression in HCC tissues. The results of MTT and Transwell assay showed that miR-23c inhibition restored the inhibitory effect of ZEB1-AS1 knockdown on HCC cells proliferation and invasion.
As a ceRNA, lncRNA ZEB1-AS1 may play a vital role in inhibiting HCC progression through miR-23c, which will provide new clues and theoretical basis for the HCC diagnosis and treatment.
据报道，长链非编码 RNA（lncRNA）锌指 E 盒结合同源盒 1 反义 1（ZEB1-AS1）是多种癌症（包括肝细胞癌[HCC]）的癌基因。我们研究了 ZEB1-AS1 作为竞争性内源性 RNA（ceRNA）与 miR-23c 结合在 HCC 细胞增殖和侵袭中的作用和机制。
实时定量聚合酶链反应（QRT-PCR）用于检测 HCC 组织和细胞中的 ZEB1-AS1 和 miR-23c 表达。双荧光素酶报告基因实验检测 miR-23c 和 ZEB1-AS1 的靶向调节。我们还通过 Spearman 相关分析对 HCC 组织中它们的表达进行了相关性分析。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）检测法用于检测肝癌细胞的增殖。通过 Transwell 测定法评估细胞侵袭。
QRT-PCR 结果表明，ZEB1-AS1 在 HCC 组织和细胞系中上调，miR-23c 下调。ZEB1-AS1 敲低阻碍了 HCC 细胞的增殖和侵袭。双荧光素酶报告基因实验表明，miR-23c 是 ZEB1-AS1 的靶标，并且 ZEB1-AS1 与 HCC 组织中 miR-23c 的表达呈显著负相关。MTT 和 Transwell 实验结果表明，抑制 miR-23c 恢复了 ZEB1-AS1 敲低对 HCC 细胞增殖和侵袭的抑制作用。
作为 ceRNA，lncRNA ZEB1-AS1 可能通过 miR-23c 抑制 HCC 进展中发挥重要作用，为 HCC 的诊断和治疗提供了新的线索和理论依据。
