Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; Department of Animal Husbandry and Veterinary Medicine, College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404100, China.
Department of Animal Husbandry and Veterinary Medicine, College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404100, China; Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
J Dairy Sci. 2021 Jul;104(7):8177-8187. doi: 10.3168/jds.2020-19761. Epub 2021 Apr 15.
Left displaced abomasum (LDA) leads to substantial changes in the metabolism of dairy cows. Surgical correction of LDA can rapidly improve the health of cows; however, changes in metabolism following surgery are rarely described. To investigate the changes of plasma metabolome in cows with LDA before and after surgical correction, blood samples were collected from 10 healthy postpartum cows and 10 cows with LDA on the day of diagnosis, then again from the LDA cows 14 d after surgery. Serum nonesterified fatty acid, β-hydroxybutyric acid, cortisol and histamine concentration, and antioxidant enzyme (superoxide dismutase and glutathione peroxidase) activities were evaluated, and the metabolic profile in plasma was analyzed using ultra-high-performance liquid chromatography time-of-flight mass spectrometry. The results demonstrated that cows with LDA experienced severe negative energy balance and oxidative stress, which can be improved by surgical correction. The metabolic profile was analyzed using multidimensional and univariate statistical analyses, and different metabolites were identified. In total, 102 metabolites differed between cows with LDA and healthy cows. After surgical correction, 65 metabolites changed in cows with LDA, compared with these cows during the LDA event. Following surgical correction, AA levels tended to increase, and lipid levels tended to decrease in cows with LDA. Pathway analysis indicated marked changes in linoleic acid metabolism, Arg biosynthesis, and Gly, Ser, and Thr metabolism in cows at the onset of LDA and following surgical correction. Surgical treatment reversed the changes in AA and lipid metabolism in cows with LDA.
左方变位真胃（LDA）会导致奶牛代谢发生重大变化。LDA 的手术矫正可以迅速改善奶牛的健康状况；然而，手术后代谢的变化很少被描述。为了研究 LDA 奶牛在手术前后血浆代谢组的变化，从 10 头健康产后奶牛和 10 头 LDA 奶牛的诊断日采集血液样本，然后再次从 LDA 奶牛手术后 14 天采集。评估血清非酯化脂肪酸、β-羟丁酸、皮质醇和组胺浓度以及抗氧化酶（超氧化物歧化酶和谷胱甘肽过氧化物酶）活性，并使用超高效液相色谱飞行时间质谱分析血浆中的代谢谱。结果表明，LDA 奶牛经历了严重的负能量平衡和氧化应激，手术矫正可以改善这种情况。使用多维和单变量统计分析对代谢谱进行分析，鉴定出不同的代谢物。共有 102 种代谢物在 LDA 奶牛和健康奶牛之间存在差异。手术后，与 LDA 期间相比，65 种代谢物在 LDA 奶牛中发生了变化。手术后，LDA 奶牛的 AA 水平趋于增加，脂质水平趋于降低。途径分析表明，LDA 奶牛发病时和手术后，亚油酸代谢、精氨酸生物合成以及甘氨酸、丝氨酸和苏氨酸代谢发生了明显变化。手术治疗逆转了 LDA 奶牛 AA 和脂质代谢的变化。
