National Research Base of Intelligent Manufacturing Service, Chongqing Technology and Business University, Chongqing, 400067, China; Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China; Environmental Engineering Technology Research Center, Chongqing Academy of Ecological and Environmental Sciences, Chongqing, 401147, China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China.
Environ Res. 2021 Jun;197:111178. doi: 10.1016/j.envres.2021.111178. Epub 2021 Apr 16.
The adaptation mechanism of a wild type (WT) and resistant type (Re) strain of the aerobic denitrifier Enterobacter cloacae strain HNR to short-term ZnO nanoparticle (NP) stresses was investigated. The results showed that Re maintained higher nitrite reductase (NIR) and nitrate reductase (NR) activities and showed lower increment of reactive oxygen species (ROS) than WT, under ZnO NP stresses. The affinity constant (K) of WT to Zn was 5.06 times that of Re, indicating that Re was more repulsive to Zn released by ZnO NPs. Transcriptomic analysis revealed that the up-regulation of the nitrogen metabolism of Re helped maintain NIR and NR activities, that the enhancement of purine metabolism lowered the intracellular ROS increment, and that the up-regulation of cationic antimicrobial peptide resistance contributed to the lower K of Re to Zn. These findings provided new insights into the adaptation mechanism of aerobic denitrifying bacteria to ZnO NPs.
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