Zuo Jinjiao, Cao Yanan, Wang Zhisheng, Shah Abid Ullah, Wang Wenlei, Dai Chen, Chen Mingjia, Lin Jian, Yang Qian
College of Life Sciences, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu 210095, PR China.
College of Veterinary Medicine, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu 210095, PR China.
Genomics. 2021 Jul;113(4):1719-1732. doi: 10.1016/j.ygeno.2021.04.027. Epub 2021 Apr 16.
Dendritic cells are first guard to defend avian infectious bronchitis virus (IBV) infection and invasion. While IBV always suppress dendritic cells and escape the degradation and presentation, which might help viruses to transfer and migrant. Initially, we compared two IBV's function in activating avian bone marrow dendritic cells (BMDCs) and found that both IBV (QX and M41) did not significantly increase surface marker of avian BMDCs. Moreover, a significant decrease of mA modification level in mRNA, but an increased in the ut RNA were observed in avian BMDCs upon the prevalent IBV (QX) infection. Further study found that both non-structural protein 7 (NSP7) and NSP16 inhibited the maturation and cytokines secretion of BMDCs, as well as their antigen-presentation ability. Lastly, we found that gga-miR21, induced by both NSP7 and NSP16, inhibited the antigen presentation of avian BMDCs. Taken together, our results illustrated how IBV inhibited the antigen-presentation of avian DCs.
树突状细胞是抵御禽传染性支气管炎病毒（IBV）感染和入侵的第一道防线。然而，IBV总是抑制树突状细胞并逃避降解和呈递，这可能有助于病毒转移和迁移。最初，我们比较了两种IBV在激活禽骨髓树突状细胞（BMDCs）方面的功能，发现两种IBV（QX和M41）均未显著增加禽BMDCs的表面标志物。此外，在流行的IBV（QX）感染后，禽BMDCs中mRNA的mA修饰水平显著降低，但ut RNA增加。进一步研究发现，非结构蛋白7（NSP7）和NSP16均抑制BMDCs的成熟和细胞因子分泌，以及它们的抗原呈递能力。最后，我们发现由NSP7和NSP16诱导的gga-miR21抑制了禽BMDCs的抗原呈递。综上所述，我们的结果阐明了IBV如何抑制禽树突状细胞的抗原呈递。
