College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Poult Sci. 2023 May;102(5):102620. doi: 10.1016/j.psj.2023.102620. Epub 2023 Mar 3.
The gamma-coronavirus infectious bronchitis virus (IBV) has a high mutation rate and mainly invades the respiratory mucosa, making it difficult to prevent and causing great economic losses. Nonstructural protein 16 (NSP16) of IBV QX also not only plays an indispensable role in virus invading but also might hugely influence the antigen's recognition and presentation ability of host BMDCs. Hence, our study tries to illustrate the underline mechanism of how NSP16 influences the immune function of BMDCs. Initially, we found that NSP16 of the QX strain significantly inhibited the antigen presentation ability and immune response of mouse BMDCs, which was stimulated by Poly (I:C) or AIV RNA. Besides mouse BMDCs, we also found that NSP16 of the QX strain also significantly stimulated the chicken BMDCs to activate the interferon signaling pathway. Furthermore, we preliminarily demonstrated that IBV QX NSP16 inhibits the antiviral system by affecting the antigen-presenting function of BMDCs.
γ 冠状病毒传染性支气管炎病毒（IBV）具有很高的突变率，主要侵犯呼吸道黏膜，难以预防，造成巨大的经济损失。IBV QX 的非结构蛋白 16（NSP16）不仅在病毒入侵中发挥不可或缺的作用，而且可能极大地影响宿主 BMDCs 的抗原识别和呈递能力。因此，我们的研究试图阐明 NSP16 影响 BMDCs 免疫功能的潜在机制。首先，我们发现 QX 株的 NSP16 显著抑制了由 Poly（I:C）或 AIV RNA 刺激的小鼠 BMDCs 的抗原呈递能力和免疫反应。除了小鼠 BMDCs 外，我们还发现 QX 株的 NSP16 也显著刺激了鸡 BMDCs 激活干扰素信号通路。此外，我们初步表明，IBV QX NSP16 通过影响 BMDCs 的抗原呈递功能来抑制抗病毒系统。
