Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, 157 71, Greece.
Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, 157 71, Greece.
Anal Chim Acta. 2021 May 15;1159:338426. doi: 10.1016/j.aca.2021.338426. Epub 2021 Mar 20.
In this paper, a 3D-printed microfluidic device is described which is suitable for sequential injection/anodic stripping voltammetric (SIA-ASV) determination of Pb(II) and Cd(II). The fluidic device is manufactured by 3D-printing in a single-step using a dual extruder 3D printer. The device is composed of a microfluidic cell (printed from a non-conductive polylactic acid (PLA) filament) and of 3 electrodes (printed from a conductive carbon-loaded PLA filament) which are integrated within the fluidic cell. During the preconcentration step, a zone of the sample (containing the target cations) and a zone of Bi(III) solution are mixed on-line and the cations are reduced on the working electrode forming a bismuth alloy. The detection step involves a voltametric scan in static solution, in which the accumulated metals are oxidized while the oxidation current is monitored. After the optimization of the relevant parameters, the limit of detection is 0.38 μg L for Pb(II) and 0.57 μg L for Cd(II), while the within-device repeatability and the between-device reproducibility are lower than 4.5% (n = 8) and 9% (n = 6), respectively, for both cations at the 30 μg L level. The device was successfully applied to the simultaneous determination of Pb(II) and Cd(II) in a honey sample.
本文描述了一种适用于顺序注射/阳极溶出伏安法（SIA-ASV）测定 Pb(II) 和 Cd(II) 的 3D 打印微流控装置。该流体装置通过使用双挤出 3D 打印机进行一步 3D 打印制造而成。该装置由微流控池（由非导电聚乳酸（PLA）长丝打印而成）和 3 个电极（由导电负载碳的 PLA 长丝打印而成）组成，这些电极集成在微流控池内。在预浓缩步骤中，样品的一个区域（含有目标阳离子）和一个铋（III）溶液区域在线混合，阳离子在工作电极上还原形成铋合金。检测步骤涉及在静态溶液中的伏安扫描，在此过程中，在监测氧化电流的同时，将累积的金属氧化。在优化相关参数后，对于 Pb(II)，检测限为 0.38μg L，对于 Cd(II)，检测限为 0.57μg L，而对于这两种阳离子，在 30μg L 水平下，装置内重复性和装置间重现性均低于 4.5%（n=8）和 9%（n=6）。该装置成功应用于蜂蜜样品中 Pb(II) 和 Cd(II) 的同时测定。
