Abbas Tanveer, Sara Bisma, Shoaib Affhan
Department of Microbiology, University of Karachi, Karachi, Pakistan.
Barrett Hodgson University, Karachi, Pakistan.
Pak J Pharm Sci. 2020 Nov;33(6):2557-2565.
From the ancient times, the use of plants is considered as a cure for many diseases and now, they are being used as a new resource for producing agents that could act as alternative to antibiotics. Current work was carried to investigate the antimicrobial potential of 42 different aqueous plant extracts and oils against Staphylococcus aureus, Candida albicans and Pseudomonas aeruginosa. In vitro antimicrobial activity of different concentrations (1.50mg/ml and 0.75mg/ml) of plant fractions was investigated against three clinical isolates: S. aureus, C. albicans and P. aeruginosa by agar well diffusion assay. Minimum Inhibitory Concentrations (MICs) and Minimum Bactericidal Concentrations (MBCs) were evaluated by broth dilution and plating method respectively. Among 42 plant fractions, methanol fractions of Cassia fistula leaves and flowers were active against S. aureus. Hexane fractions of Ixora coccinea stalk and leaves, methanol fractions of C. fistula flowers and chloroform fraction of C. fistula leaves were active against C. albicans. Methanol fractions of I. coccinea leaves and stalk were active against P. aeruginosa. The MICs of active fractions were found to be 0.45mg/ml and 0.6mg/ml for P. aeruginosa, S. aureus and C. albicans. The MBCs were found to be 1.50mg/ml for P. aeruginosa, 0.75mg/ml for S. aureus and 1.50mg/ml and 0.75mg/ml for C. albicans. In antibiotic susceptibility testing, all isolates were found to be sensitive to their respective antibiotics.
从古代起，植物的使用就被视为治疗多种疾病的方法，如今，它们正被用作生产可替代抗生素的药物的新资源。目前开展的工作是研究42种不同植物水提取物和油对金黄色葡萄球菌、白色念珠菌和铜绿假单胞菌的抗菌潜力。通过琼脂孔扩散法，研究了不同浓度（1.50mg/ml和0.75mg/ml）的植物提取物对三种临床分离株：金黄色葡萄球菌、白色念珠菌和铜绿假单胞菌的体外抗菌活性。分别采用肉汤稀释法和平板接种法评估最低抑菌浓度（MICs）和最低杀菌浓度（MBCs）。在42种植物提取物中，决明子叶和花的甲醇提取物对金黄色葡萄球菌有活性。龙船花茎和叶的己烷提取物、决明子花的甲醇提取物和决明子叶的氯仿提取物对白色念珠菌有活性。龙船花叶和茎的甲醇提取物对铜绿假单胞菌有活性。发现活性提取物对铜绿假单胞菌、金黄色葡萄球菌和白色念珠菌的MICs分别为0.45mg/ml和0.6mg/ml。发现铜绿假单胞菌的MBCs为1.50mg/ml，金黄色葡萄球菌为0.75mg/ml，白色念珠菌为1.50mg/ml和0.75mg/ml。在抗生素敏感性测试中，发现所有分离株对其各自的抗生素敏感。
