Loredo Axel, Wang Lushun, Wang Shichao, Xiao Han
Department of Chemistry, Rice University, 6100 Main Street, Houston, Texas, 77005.
Department of Biosciences, Rice University, 6100 Main Street, Houston, Texas, 77005.
Dyes Pigm. 2021 Feb;186. doi: 10.1016/j.dyepig.2020.109014. Epub 2020 Nov 20.
By performing a single-atom replacement within common fluorophores, we have developed a facile and general strategy to prepare a broad-spectrum class of colorimetric and fluorogenic probes for the selective detection of mercury ions in aqueous environments. Thionation of carbonyl groups from existing fluorophore cores results in a great reduction of fluorescence quantum yield and loss of fluorescence emission. The resulting thiocaged probes are efficiently desulfurized to their oxo derivatives in the presence of mercury ions, leading to pronounced changes in chromogenic and fluorogenic signals. Because these probes exhibit high selectivity, excellent sensitivity, good membrane-permeability, and rapid responses towards mercury ions, they are suitable for visualization of mercury in both aqueous and intracellular environments.
通过在常见荧光团内进行单原子替换，我们开发了一种简便通用的策略，用于制备一类广谱的比色和荧光探针，以选择性检测水环境中的汞离子。现有荧光团核心羰基的硫代化导致荧光量子产率大幅降低和荧光发射丧失。所得的硫代笼状探针在汞离子存在下能有效地脱硫生成其氧代衍生物，从而导致显色和荧光信号发生显著变化。由于这些探针具有高选择性、优异的灵敏度、良好的膜通透性以及对汞离子的快速响应，它们适用于水相和细胞内环境中汞的可视化检测。
