Wang Yen-Yun, Fang Pen-Tzu, Su Chang-Wei, Chen Yuk-Kwan, Huang Joh-Jong, Huang Ming-Yii, Yuan Shyng-Shiou F
School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 807377, Taiwan, R.O.C.
Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung 807377, Taiwan, R.O.C.
Oncol Lett. 2021 Jun;21(6):450. doi: 10.3892/ol.2021.12711. Epub 2021 Apr 7.
Oral cancer is the fourth most common type of cancer among males in Taiwan, and the prognosis for patients with advanced-stage oral squamous cell carcinoma (OSCC) remains poor. The present study investigated the prognostic value of three DNA repair genes, namely excision repair cross-complementing group 1 (ERCC1), ERCC2 and X-ray repair cross-complementing group 1 (XRCC1) in OSCC. The protein expression levels of XRCC1, ERCC1 and ERCC2 in oral cell lines were analyzed via western blotting and immunohistochemistry using samples from 98 patients with biopsy-proven OSCC, while the χ test was used to analyze the clinicopathological association. Kaplan-Meier estimates were used to determine the prognostic value of XRCC1, ERCC1 and ERCC2 for overall survival, and the log-rank test was used to evaluate the significance of differences. Multivariate analysis revealed a positive association between ERCC2 expression and OSCC recurrence (19.64-fold; 95% CI, 5.00-77.1; P<0.001). In addition, the high protein expression levels of XRCC1, ERCC1 and ERCC2 were associated with poor disease-free and overall survival rates. Therefore, the present study suggested that high ERCC2 expression may be a risk factor for OSCC recurrence.
口腔癌是台湾男性中第四大常见癌症类型，晚期口腔鳞状细胞癌（OSCC）患者的预后仍然很差。本研究调查了三种DNA修复基因，即切除修复交叉互补基因1（ERCC1）、ERCC2和X射线修复交叉互补基因1（XRCC1）在OSCC中的预后价值。使用98例经活检证实为OSCC患者的样本，通过蛋白质印迹法和免疫组织化学分析口腔细胞系中XRCC1、ERCC1和ERCC2的蛋白质表达水平，同时使用χ检验分析临床病理相关性。采用Kaplan-Meier估计法确定XRCC1、ERCC1和ERCC2对总生存期的预后价值，并使用对数秩检验评估差异的显著性。多变量分析显示ERCC2表达与OSCC复发之间存在正相关（19.64倍；95%CI，5.00-77.1；P<0.001）。此外，XRCC1、ERCC1和ERCC2的高蛋白表达水平与无病生存率和总生存率低相关。因此，本研究表明ERCC2高表达可能是OSCC复发的一个危险因素。
