评估溶剂对DPPH反应性的影响以测定油基质中的抗氧化活性。
Evaluation of solvent effects on the DPPH reactivity for determining the antioxidant activity in oil matrix.
作者信息
La JinWook, Kim Mi-Ja, Lee JaeHwan
机构信息
Department of Food Science and Biotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do 16419 Republic of Korea.
Department of Food and Nutrition, Kangwon National University, Samcheok, Republic of Korea.
出版信息
Food Sci Biotechnol. 2021 Mar 20;30(3):367-375. doi: 10.1007/s10068-020-00874-9. eCollection 2021 Mar.
UNLABELLED
Reactivity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical in methanol, ethanol, isopropanol, isooctane, and ethyl acetate, was evaluated to assess the antioxidant capabilities in medium chain triacylglycerol. DPPH loss values were obtained over 30 min, with sampling every 5 min. Even the same concentration of antioxidants showed different DPPH reactivity depending on solvent. In methanol, 5 min was enough for α-tocopherol to react with DPPH, whereas BHT did not react with DPPH even after 30 min. Gallate series showed higher DPPH reactivity than TBHQ, sesamol, or BHA in methanol, while lower reactivity in isooctane. Antioxidants in ethanol and isopropanol reacted with DPPH less efficiently compared to those in methanol, the exception being sesamol. DPPH reactivity of gallate series in isooctane was lower than that of sesamol, TBHQ, and α-tocopherol. Combinatorial usage of methanol and isooctane for DPPH reactivity could provide reliable information on the antioxidant capacities of chemicals in edible oils.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-020-00874-9.
未标注
评估了2,2-二苯基-1-苦基肼（DPPH）自由基在甲醇、乙醇、异丙醇、异辛烷和乙酸乙酯中的反应活性，以评估中链甘油三酯中的抗氧化能力。在30分钟内获取DPPH损失值，每5分钟取样一次。即使是相同浓度的抗氧化剂，其DPPH反应活性也会因溶剂而异。在甲醇中，α-生育酚与DPPH反应5分钟就足够了，而丁基羟基甲苯（BHT）即使在30分钟后也不与DPPH反应。没食子酸系列在甲醇中显示出比特丁基对苯二酚（TBHQ）、芝麻酚或丁基羟基茴香醚（BHA）更高的DPPH反应活性，而在异辛烷中反应活性较低。与甲醇中的抗氧化剂相比，乙醇和异丙醇中的抗氧化剂与DPPH的反应效率较低，芝麻酚除外。没食子酸系列在异辛烷中的DPPH反应活性低于芝麻酚、TBHQ和α-生育酚。将甲醇和异辛烷用于DPPH反应活性的组合使用可以提供有关食用油中化学物质抗氧化能力的可靠信息。
补充信息
在线版本包含可在10.1007/s10068-020-00874-9获取的补充材料。
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