ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kiev, Ukraine.
Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine.
Naturwissenschaften. 2023 Apr 18;110(3):15. doi: 10.1007/s00114-023-01844-x.
Metallic nanoparticles of different compositions have already found numerous applications in various branches of industry, agriculture, and medicine. Given the well-known antibacterial activity of Ag, silver nanoparticles (AgNPs) are constantly being investigated for their promising ability to fight antibiotic-resistant pathogens. A promising candidate for AgNPs biosynthesis is chili pepper Capsicum annuum, cultivated worldwide and known for accumulating significant amounts of active substances. Phytochemical screening of aqueous extract of C. annuum pericarps demonstrated accumulation of 4.38 mg/g DW of total capsaicinoids, 14.56 mg GAE/g DW of total phenolic compounds, 1.67 mg QE/g DW of total flavonoids, and 1.03 mg CAE/g DW of total phenolic acids. All determined aromatic compounds carry various active functional groups, which effectively participate in the biosynthesis of AgNPs and are characterized by high antioxidant potential. Therefore, the present research focused on the facile, quick, and effective procedure for the biosynthesis of AgNPs, which were analyzed for their morphology such as shape and size through UV-visible, Fourier-transform infrared spectroscopy (FTIR) assays, and scanning electron microscopy. We found that the AgNPs biosynthesis resulted in changes in FTIR spectra, depicting the rearrangement of numerous functional groups, while the nanoparticles themselves were shown to be stable, spherical, 10-17 nm in size. Also we investigated the antibacterial properties of biosynthesized AgNPs, obtained with C. annuum fruit extracts, against a common phytopathogen Clavibacter michiganensis subsp. michiganensis. As was shown by zone inhibition assay, AgNPs showed dose-dependent 5.13-6.44 cm antibacterial activity, greatly exceeding the 4.98 cm inhibition area, produced by the precursor salt, AgNO.
不同组成的金属纳米粒子已经在工业、农业和医学的各个领域得到了广泛的应用。鉴于银的众所周知的抗菌活性，银纳米粒子（AgNPs）因其对抗生素耐药病原体的有希望的抗菌能力而不断被研究。辣椒（Capsicum annuum）是一种很有前途的 AgNPs 生物合成候选物，在世界各地都有种植，并且以积累大量活性物质而闻名。对辣椒果皮水提物的植物化学筛选表明，总辣椒素积累量为 4.38 mg/g DW，总酚含量为 14.56 mg GAE/g DW，总类黄酮含量为 1.67 mg QE/g DW，总酚酸含量为 1.03 mg CAE/g DW。所有确定的芳香族化合物都携带各种活性官能团，这些官能团有效地参与了 AgNPs 的生物合成，并具有很高的抗氧化潜力。因此，本研究集中于 AgNPs 的生物合成，该方法简便、快速且有效，通过紫外-可见分光光度法、傅里叶变换红外光谱（FTIR）分析和扫描电子显微镜分析了 AgNPs 的形貌，如形状和尺寸。我们发现，AgNPs 的生物合成导致了 FTIR 光谱的变化，描绘了许多功能基团的重新排列，而纳米粒子本身则表现出稳定的、球形的，尺寸为 10-17nm。我们还研究了用辣椒果实提取物获得的生物合成 AgNPs 对常见植物病原体茄科劳尔氏菌（Clavibacter michiganensis subsp. michiganensis）的抗菌性能。抑菌圈试验表明，AgNPs 表现出剂量依赖性的 5.13-6.44cm 的抗菌活性，大大超过了前体盐 AgNO3 产生的 4.98cm 的抑制区域。
