Yoon Jae-Hyun, Bae Young-Min, Song Hana, Lee Soyul, Moon Sung-Kwon, Oh Se-Wook, Lee Sun-Young
Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, 55365 Republic of Korea.
Department of Food and Nutrition, Chung-Ang University, Seodong-daero, 4726 Anseong-si, Gyeonggi-do Republic of Korea.
Food Sci Biotechnol. 2021 Feb 6;30(3):475-485. doi: 10.1007/s10068-021-00877-0. eCollection 2021 Mar.
This study was undertaken to develop enhanced selective media for detection of in oysters. Primarily, tryptic soy agar (TSA) was supplemented with 4.5-5% NaCl, 0.1-0.5% oxgall, and/or 1-2% sodium citrate, and adjusted to pH 8-9. A total of 21 spp., 24 indicators, and 26 food-borne isolates were streaked on the modified media, followed by 24 h of incubation at 37 °C. While all the indicators and isolates failed to grow on TSA containing 5% NaCl, 0.5% oxgall, and 2% sodium citrate (TSA; pH 9), was culturable on this selective medium. Particularly, the ability of TSA to quantify . in oysters was superior to thiosulphate citrate bile salts sucrose (TCBS) agar. distinctly produced its white-yellowish, round, and edge-pointed colony on TSA. TSAO with high selectivity potentials over TCBS may be a promising alternative for detection of . in seafoods or natural reservoirs.
The online version contains supplementary material available at (10.1007/s10068-021-00877-0).
本研究旨在开发用于检测牡蛎中[具体微生物名称未给出]的强化选择性培养基。首先，在胰蛋白胨大豆琼脂（TSA）中添加4.5 - 5%的氯化钠、0.1 - 0.5%的牛胆盐和/或1 - 2%的柠檬酸钠，并将pH值调至8 - 9。总共将21种[具体微生物名称未给出]菌株、24种指示菌和26种食源性病原体分离株接种在改良培养基上，然后在37℃下培养24小时。虽然所有指示菌和分离株在含有5%氯化钠、0.5%牛胆盐和2%柠檬酸钠（TSA；pH 9）的TSA上均无法生长，但[具体微生物名称未给出]能在这种选择性培养基上培养。特别是，TSA定量牡蛎中[具体微生物名称未给出]的能力优于硫代硫酸盐柠檬酸盐胆盐蔗糖（TCBS）琼脂。[具体微生物名称未给出]在TSA上明显产生其黄白色、圆形且边缘尖锐的菌落。与TCBS相比具有高选择性潜力的TSAO可能是检测海鲜或天然水体中[具体微生物名称未给出]的一种有前景的替代方法。
在线版本包含可在(10.1007/s10068 - 021 - 00877 - 0)获取的补充材料。
