Northwest Fisheries Science Center, National Oceanic and Atmospheric Administration, Seattle, WA, 98112, USA; School of Oceanography, University of Washington, Seattle, WA, 98195, USA.
Environ Microbiol. 2014 Apr;16(4):1019-28. doi: 10.1111/1462-2920.12246. Epub 2013 Sep 12.
Over a 1-year period, bi-monthly estuarine surface water and plankton samples (63-200 and > 200 μm fractions) were assayed by polymerase chain reaction for the prevalence of total Vibrio parahaemolyticus, V. vulnificus and V. cholerae and select genes associated with clinical strains found in each species. Neither temperature nor plankton abundance was a significant correlate of total V. parahaemolyticus; however, the prevalence of genes commonly associated with clinical strains (trh, tdh, ORF8) increased with temperature and copepod abundance (P < 0.05). The prevalence of total V. vulnificus and the siderophore-related viuB gene also increased with temperature and copepod and decapod abundance (P < 0.001). Temperature and copepod abundance also covaried with the prevalence of V. cholerae (P < 0.05), but there was no significant relationship with ctxA or other genes commonly found in clinical strains. Results show that genes commonly associated with clinical Vibrio strains were more frequently detected in association with chitinous plankton. We conclude that V. parahaemolyticus, V. vulnificus, V. cholerae and subpopulations that harbour genes common to clinical strains respond distinctly to seasonal changes in temperature as well as shifts in the taxonomic composition of discrete plankton fractions.
在为期一年的时间里，通过聚合酶链反应对半年度河口地表水和浮游生物样本（63-200μm 和>200μm 级分）进行了总副溶血性弧菌、创伤弧菌和霍乱弧菌的流行情况检测，并检测了与每种物种中发现的临床株相关的选定基因。温度和浮游生物丰度均与总副溶血性弧菌无显著相关性；然而，与临床株相关的常见基因（trh、tdh、ORF8）的流行率随温度和桡足类丰度的增加而增加（P<0.05）。总创伤弧菌和与铁载体相关的viuB 基因的流行率也随温度和桡足类和十足类丰度的增加而增加（P<0.001）。温度和桡足类丰度也与霍乱弧菌的流行率相关（P<0.05），但与 ctxA 或临床株中常见的其他基因没有显著关系。结果表明，与临床副溶血性弧菌菌株相关的基因更频繁地与带壳浮游生物相关联被检测到。我们得出结论，携带与临床菌株相关基因的副溶血性弧菌、创伤弧菌、霍乱弧菌和亚群对温度的季节性变化以及离散浮游生物种群组成的变化有明显的反应。
