Nakamoto João Carlos, Wataya Erick Yoshio, Nakamoto Hugo Alberto, Santos Gustavo Bispo, Ribaric Ivan, Herrera Ana K A, Faria José C M
Plast Reconstr Surg Glob Open. 2021 Apr 15;9(4):e3514. doi: 10.1097/GOX.0000000000003514. eCollection 2021 Apr.
We aimed to evaluate the use of nerve allograft preserved in glycerol. We compared the efficiency of glycerol-preserved allografts with autogenous nerve grafting, cryopreserved grafts, and detergent-processed grafts in the axonal regeneration. Secondarily, we evaluated the effectiveness of each preservation method in maintaining the extracellular matrix free of cellular components.
This was a prospective experimental, longitudinal, unblinded, nonrandomized, controlled animal model study. Three different allograft preservation techniques for the repair of sciatic nerve injuries were compared, including cold preservation, glycerol preservation, and detergent preservation. Functional assessment was performed, and histomorphometric analyses were further performed, which enabled the allograft structure evaluation and an estimation of the nerve regeneration efficacy based on the myelinated axons count and on their diameters.
After the 14 week, all groups were already balanced and similar ( = 0.265): all groups present near-zero SFIs, thus confirming their efficiency in promoting nerve regeneration. In the histomorphometric evaluations, all groups were equivalent, presenting a similar efficiency in nerve regeneration ( = 0.716 and = 0.577, respectively). Similarly, histomorphometric evaluations showed a reduction in the number of axons and in their diameters, but none of them effectively eliminated all cellular debris. Comparing the groups with each other, the groups preserved in glycerol and detergent solution were similar, both presenting better results than the cooled group.
By evaluating the presence of cell debris after the treatment using glycerol, it was found to be similar to the treatment using detergent and significantly better than the cold-preservation treatment.
我们旨在评估甘油保存的神经同种异体移植物的应用。我们比较了甘油保存的同种异体移植物与自体神经移植、冷冻保存的移植物以及经去污剂处理的移植物在轴突再生方面的效率。其次，我们评估了每种保存方法在维持无细胞成分的细胞外基质方面的有效性。
这是一项前瞻性实验性、纵向、非盲、非随机、对照动物模型研究。比较了三种不同的用于修复坐骨神经损伤的同种异体移植物保存技术，包括冷藏保存、甘油保存和去污剂保存。进行了功能评估，并进一步进行了组织形态计量学分析，这使得能够基于有髓轴突计数及其直径对同种异体移植物结构进行评估并估计神经再生功效。
14周后，所有组已经达到平衡且相似（P = 0.265）：所有组的坐骨神经功能指数（SFI）接近零，从而证实了它们在促进神经再生方面的效率。在组织形态计量学评估中，所有组相当，在神经再生方面表现出相似的效率（分别为P = 0.716和P = 0.577）。同样，组织形态计量学评估显示轴突数量及其直径减少，但没有一种方法能有效消除所有细胞碎片。相互比较各小组，甘油保存组和去污剂溶液保存组相似，两者的结果均优于冷藏组。
通过评估使用甘油处理后细胞碎片的存在情况，发现其与使用去污剂处理的情况相似，且明显优于冷藏处理。
