Muto Nilton Akio, Hamoy Moisés, Rodrigues Lucas David Cristian, Teixeira Bruno Brito, Santos Almeida Adrielle Felicia, de Castro Navegantes Thamires, de Sousa Ferreira de Sá Vaniza Sheila, de Moraes Brenda Pinto, do Vale Medeiros João Paulo, Dos Santos Yasmin Amorim, da Rocha Claúdia Quintino, de Mello Vanessa Joia, Rogez Hervé
UFPA & Centre for Valorization of Amazonian Bioactive Compounds (CVACBA), Belém, Pará, Brazil.
Laboratory of Pharmacology and Toxicology of Natural Products, ICB-UFPA, Brazil.
Toxicol Rep. 2021 Mar 31;8:829-838. doi: 10.1016/j.toxrep.2021.03.024. eCollection 2021.
The biological and pharmacological properties of natural polyphenols of the extract of stone (EEOS) are associated with the central nervous system (CNS). To investigate the sedative and myorelaxant activity of EEOS , this study aimed to present the myorelaxant and sedative effects of EEOS in Wistar rats using spontaneous locomotor activity and motor electrophysiology. A total of 108 animals were used in the following experiments: a) behavioral tests (n = 27); b) electromyographic recordings of skeletal muscle (n = 27); c) respiratory muscle activity recordings (n = 27); d) cardiac muscle activity recordings (n = 27). The behavioral characteristics were measured according to the latency time of onset, the transient loss of posture reflex and maximum muscle relaxation. Electrodes were implanted in the gastrocnemius muscle and in the tenth intercostal space for electromyographic (EMG) signal capture to record muscle contraction, and in the D2 lead for electrocardiogram acquisition. After using the 300 mg/kg dose of EEOS intraperitoneally, a myorelaxant activity exhibited a lower frequency of contractility with an amplitude pattern of low and short duration at gastrocnemius muscle and intercostal muscle, which clearly describes a myorelaxant activity and changes in cardiac activity. The present report is so far the first study to demonstrate the myorelaxant activity of this extract, indicating an alternative route for açai stone valorization and its application in pharmaceutical fields.
巴西莓籽提取物（EEOS）中天然多酚的生物学和药理学特性与中枢神经系统（CNS）相关。为研究EEOS的镇静和肌松活性，本研究旨在利用自发运动活动和运动电生理学方法，呈现EEOS对Wistar大鼠的肌松和镇静作用。以下实验共使用了108只动物：a）行为测试（n = 27）；b）骨骼肌肌电图记录（n = 27）；c）呼吸肌活动记录（n = 27）；d）心肌活动记录（n = 27）。根据起效潜伏期、姿势反射的短暂丧失和最大肌肉松弛程度来测量行为特征。将电极植入腓肠肌和第十肋间间隙以采集肌电图（EMG）信号来记录肌肉收缩，并在D2导联采集心电图。腹腔注射300 mg/kg剂量的EEOS后，腓肠肌和肋间肌的肌松活性表现为收缩频率降低，幅度模式为低且持续时间短，这清楚地描述了肌松活性以及心脏活动的变化。本报告是迄今为止第一项证明该提取物具有肌松活性的研究，为巴西莓籽的增值利用及其在制药领域的应用指明了一条新途径。
