Al-Abbasi Fahad A, Kumar Vikas, Anwar Firoz
Department of Biochemistry, Faculty of Science, King Abdulaziz University, Saudi Arabia.
Department of Pharmaceutical Sciences, Shalom Institute of Health and Allied Sciences (SIHAS), Sam Higginbottom University of Agriculture, Technology & Sciences (SHUATS), Allahabad, India.
Toxicol Rep. 2020 Jun 18;7:788-794. doi: 10.1016/j.toxrep.2020.06.004. eCollection 2020.
Diazepam is a medicine of the family benzodiazepine, used to treat various CNS disorders. To date, no study is available for biochemical analysis of diazepam in cardiac dysfunction. This study aimed to determine the effect of diazepam in stress-induced cardiac dysfunctions in rats. Male Wistar Albino rats were divided into four groups with six animals in each group for 90 days of the experimental protocol. Group1 served as a Normal Control (NC), Groups 2, as a Disease Control (DC), Group 3 as a Diazepam Control (DIC), and Group 4 as a Disease + Diazepam Treatment (DDT). Disease Control and Disease + Diazepam Treatment animals exposed to regular stress by forced swimming exercise method for 3 months. Diazepam Control and Disease + Diazepam Treatment received 5 mg/kg/p.o the daily dose of diazepam. At the end of the protocol, animals were sacrificed, heart preserved, blood collected, and utilized for biochemical estimations. Heart weight was increased in DC as compared to NC. Serum levels of cardiac biomarkers, creatine phosphokinase (CPK), creatine kinase-MB (CPK-MB), lactate dehydrogenase (LDH), High sensitivity C-reactive protein (hs-CRP) and troponin I (TnI) were significantly increased in DC as compared to NC. Heart tissue examined for histological changes. The altered serum levels of CPK, CPK-MB, LDH, hs-CRP, and TnI were significantly restored by the treatment of diazepam. Serum levels of Sodium, Potassium, Calcium, and Magnesium was increased in DC animals as compared to NC. The altered ionic level was also restored by the treatment of diazepam. Level of various cardiac markers and ions in the plasma were also slightly elevated in DIC. Histopathological studies are also in agreement with serological examinations and bonafide cardioprotective influences of diazepam in cardiac dysfunction. Conclusively research findings endorse the cardioprotective effect of diazepam in stress-induced cardiac dysfunction in rats.
地西泮是一种苯二氮䓬类药物，用于治疗各种中枢神经系统疾病。迄今为止，尚无关于地西泮在心脏功能障碍中的生化分析研究。本研究旨在确定地西泮对大鼠应激诱导的心脏功能障碍的影响。将雄性Wistar白化大鼠分为四组，每组六只动物，进行为期90天的实验方案。第1组作为正常对照组（NC），第2组作为疾病对照组（DC），第3组作为地西泮对照组（DIC），第4组作为疾病+地西泮治疗组（DDT）。疾病对照组和疾病+地西泮治疗组的动物通过强迫游泳运动方法接受3个月的常规应激。地西泮对照组和疾病+地西泮治疗组接受每日剂量为5mg/kg口服的地西泮。在实验方案结束时，处死动物，保存心脏，采集血液，并用于生化测定。与NC组相比，DC组的心脏重量增加。与NC组相比，DC组血清中心脏生物标志物肌酸磷酸激酶（CPK）、肌酸激酶-MB（CPK-MB）、乳酸脱氢酶（LDH）、高敏C反应蛋白（hs-CRP）和肌钙蛋白I（TnI）的水平显著升高。检查心脏组织的组织学变化。地西泮治疗可显著恢复CPK、CPK-MB、LDH、hs-CRP和TnI的血清水平变化。与NC组相比，DC组动物血清中钠、钾、钙和镁的水平升高。地西泮治疗也可恢复离子水平变化。DIC组血浆中各种心脏标志物和离子水平也略有升高。组织病理学研究也与血清学检查一致，证实了地西泮对心脏功能障碍具有真正的心脏保护作用。最终研究结果证实了地西泮对大鼠应激诱导的心脏功能障碍具有心脏保护作用。
