Giordano Liliana, Liotti Flora Marzia, Menchinelli Giulia, De Angelis Giulia, D'Inzeo Tiziana, Morandotti Grazia Angela, Sanguinetti Maurizio, Spanu Teresa, Posteraro Brunella
Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.
Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.
Front Cell Infect Microbiol. 2021 Mar 19;11:649769. doi: 10.3389/fcimb.2021.649769. eCollection 2021.
The bacteremia level as well as the administration of antibiotics before blood collection may significantly affect the recovery of bacterial pathogens from pediatric blood cultures in BacT/Alert Virtuo or Bactec FX BC systems, which remain the common techniques to diagnose bacteremia in pediatric patients. We simulated pediatric blood cultures with low or intermediate bacteremia level to evaluate BacT/Alert PF Plus and Bactec Peds Plus blood culture bottles for resin-based inactivation of 16 antibiotic-bacterium combinations. Overall, 105/192 (54.7%) of BacT/Alert PF Plus bottles and 69/192 (36.0%) of Bactec Peds Plus bottles allowed organisms to grow when exposed to antibiotics. In particular, both BacT/Alert PF Plus and Bactec Peds Plus bottles proved to be effective with piperacillin/tazobactam and or with oxacillin and methicillin-susceptible (100% growth), whereas no effectiveness was apparent with ceftriaxone and , , or or with cefepime and (0% growth). In some relevant instances (, with vancomycin and methicillin-resistant or ), BacT/Alert PF Plus bottles were superior to Bactec Peds Plus bottles. Together, these findings underscore the potentiality of resin-containing bottles to enhance diagnosis of bacteremia in pediatric patients on antimicrobial therapy. This is particularly true with one of the evaluated BC systems and with simulated intermediate bacteremia level only.
在BacT/Alert Virtuo或Bactec FX BC系统中，菌血症水平以及采血前抗生素的使用可能会显著影响从儿科血培养中分离细菌病原体，这两种系统仍是诊断儿科患者菌血症的常用技术。我们模拟了低或中度菌血症水平的儿科血培养，以评估BacT/Alert PF Plus和Bactec Peds Plus血培养瓶对16种抗生素 - 细菌组合的基于树脂的灭活作用。总体而言，当暴露于抗生素时，105/192（54.7%）的BacT/Alert PF Plus瓶和69/192（36.0%）的Bactec Peds Plus瓶允许微生物生长。特别是，BacT/Alert PF Plus和Bactec Peds Plus瓶对哌拉西林/他唑巴坦以及对苯唑西林和甲氧西林敏感菌均有效（100%生长），而对头孢曲松以及对头孢吡肟则无明显效果（0%生长）。在一些相关情况下（如万古霉素和耐甲氧西林菌），BacT/Alert PF Plus瓶优于Bactec Peds Plus瓶。总之，这些发现强调了含树脂瓶在增强接受抗菌治疗的儿科患者菌血症诊断方面的潜力。仅在评估的一种BC系统以及模拟中度菌血症水平的情况下尤其如此。
