Muñoz-Guerrero Jaider, Guerra-Sierra Beatriz E, Alvarez Javier C
Research Group in Agro-Environmental Biotechnology and Health, MICROBIOTA, Faculty of Exact Natural and Agricultural Sciences, University of Santander, Bucaramanga, Colombia.
Department of Biological Sciences, Eafit University, Medellín, Colombia.
Front Bioeng Biotechnol. 2021 Apr 1;9:650351. doi: 10.3389/fbioe.2021.650351. eCollection 2021.
is one of the causal agents of anthracnose in several crops, and of post-flowering fruit drop (PFD) in citrus and key lime anthracnose (KLA). The pathogen normally attacks flowers, causing lesions only in open flowers. Under very favorable conditions, however, it can also affect flower buds and small fruits, causing complete rotting of the fruit and a premature fall, resulting in major economic crop losses. We isolated endophytic fungi from Tahiti lime to evaluate its diversity, verify its antagonistic capacity against the phytopathogen C-100 in dual tests, and evaluate the ability of various endophytic agents to control flowers with induced anthracnose. 138 fungal isolates were obtained from 486 fragments of branches, leaves, and fruit; from which 15 species were identified morphologically. A higher isolation frequency was found in branches and leaves, with a normal level of diversity compared to other citrus species. Of the 15 morphospecies, 5 were trialed against in antagonism tests, resulting in a finding of positive inhibition. 2 endophytic fungi from the antagonism tests demonstrated high inhibition of the phytopathogen, and were thus used in tests with Tahiti lime flowers, applied in a spore solution. Spore solutions of two molecularly identified species, , and , reduced the lesions caused by the phytopathogen in these tests. The finding that these endophytes react antagonistically against may make them good candidates for further biological control research in an agroindustry that requires environmental sustainability.
是几种作物炭疽病的致病因子之一，也是柑橘和墨西哥莱檬炭疽病（KLA）中花后落果（PFD）的致病因子。该病原菌通常侵袭花朵，仅在开放的花朵中造成病斑。然而，在非常有利的条件下，它也会影响花芽和小果实，导致果实完全腐烂并过早掉落，造成重大的经济作物损失。我们从塔希提莱檬中分离内生真菌，以评估其多样性，在双培养试验中验证其对植物病原菌C - 100的拮抗能力，并评估各种内生菌剂对诱导炭疽病花朵的防治能力。从486个枝条、叶片和果实片段中获得了138株真菌分离物；通过形态学鉴定出15个物种。在枝条和叶片中发现了较高的分离频率，与其他柑橘品种相比，多样性水平正常。在15个形态种中，5个在拮抗试验中对[未提及的某种病原菌]进行了测试，结果发现有阳性抑制作用。拮抗试验中的2种内生真菌对植物病原菌表现出高度抑制作用，因此用于对塔希提莱檬花朵的[未提及的某种试验]，以孢子溶液形式施用。两种经分子鉴定的物种[未提及具体物种名称]的孢子溶液在这些[未提及的某种试验]中减少了由植物病原菌引起的病斑。这些内生菌对[未提及的某种病原菌]产生拮抗反应的发现，可能使它们成为在需要环境可持续性的农业产业中进行进一步生物防治研究的良好候选对象。
