Peng Xuan, Zhang Yanan, Wan Chunpeng, Gan Zengyu, Chen Chuying, Chen Jinyin
Jiangxi Key Laboratory for Postharvest Preservation and Non-destruction Testing of Fruits and Vegetables, College of Agriculture, Jiangxi Agricultural University, Nanchang, China.
Front Microbiol. 2022 Apr 12;13:874430. doi: 10.3389/fmicb.2022.874430. eCollection 2022.
Postharvest fungal infection can accelerate the quality deterioration of Ponkan fruit and reduce its commodity value. is the causal pathogen of blue mold in harvested citrus fruits, not only causing huge fungal decay but also leading to quality deterioration. In our preliminary study, antofine (ATF) was found to have a great potential for significant suppression of growth. However, the regulatory mechanism underpinning ATF-triggered resistance against in citrus fruit remains unclear. Here, the protective effects of ATF treatment on blue mold development in harvested Ponkan fruit involving the enhancement of ROS-scavenging system were investigated. Results showed that ATF treatment delayed blue mold development and peel firmness loss. Moreover, the increase of electrolyte leakage, O production, and malonyldialdehyde accumulation was significantly inhibited by ATF treatment. The ATF-treated Ponkan fruit maintained an elevated antioxidant capacity, as evidenced by inducted the increase in glutathione (GSH) content, delayed the declines of ascorbic acid (AsA) content and GSH/oxidized GSH ratio, and enhanced the activities of superoxide dismutase, catalase, peroxidase, and six key AsA-GSH cycle-related enzymes, along with their encoding gene expressions, thereby maintaining ROS homeostasis and reducing postharvest blue mold in harvested Ponkan fruit. Collectively, the current study revealed a control mechanism based on ATF-triggered resistance and maintenance of a higher redox state by driving AsA-GSH cycle and ROS-scavenging system in -infected Ponkan fruit.
采后真菌感染会加速椪柑果实品质劣变并降低其商品价值。 是采后柑橘类水果青霉病的致病病原体，不仅会造成巨大的真菌腐烂，还会导致品质劣变。在我们的初步研究中，发现安托芬（ATF）对 生长具有显著抑制的巨大潜力。然而，ATF引发柑橘果实对 抗性的调控机制仍不清楚。在此，研究了ATF处理对采后椪柑果实青霉病发展的保护作用，涉及活性氧清除系统的增强。结果表明，ATF处理延缓了青霉病的发展和果皮硬度的损失。此外，ATF处理显著抑制了电解质渗漏、O 产生和丙二醛积累的增加。ATF处理的椪柑果实保持了较高的抗氧化能力，这表现为诱导谷胱甘肽（GSH）含量增加、延缓抗坏血酸（AsA）含量和GSH/氧化型GSH比值的下降，并增强超氧化物歧化酶、过氧化氢酶、过氧化物酶和六种关键的AsA-GSH循环相关酶的活性，以及它们的编码基因表达，从而维持活性氧稳态并减少采后椪柑果实的青霉病。总的来说，当前研究揭示了一种基于ATF引发的抗性以及通过驱动AsA-GSH循环和活性氧清除系统在感染 的椪柑果实中维持较高氧化还原状态的控制机制。
