载紫杉醇和姜黄素的可生物降解聚合物纳米粒的治疗诊断潜力：对乳腺癌的作用。

Theranostic potential of biodegradable polymeric nanoparticles with paclitaxel and curcumin against breast carcinoma.

机构信息

Department of Biomedicine & Health Sciences, The Catholic University of Korea, Seoul 06591, Republic of Korea and Institute of Cell and Tissue Engineering, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.

Institute of Cell and Tissue Engineering, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.

出版信息

Biomater Sci. 2021 May 18;9(10):3750-3761. doi: 10.1039/d1bm00370d.

DOI:10.1039/d1bm00370d

PMID:33870964

Abstract

In this study, integrin-mediated targeting and near-infrared fluorescence (NIRF) traceable polyethylene glycol-b-poly(lactic-co-glycolic acid) (PEG-PLGA)-based polymeric nanoparticles (NPs) were prepared to investigate the effects of paclitaxel (PTX) and curcumin (CUR) combination therapy on breast cancer. Cyclic (arginine-glycine-aspartic acid-phenylalanine-lysine) (cRGDfK) was selected as a ligand for breast cancer and conjugated to the end of NPs (cRGDfK-NPs). For fluorescence imaging, sulfo-cyanine 5.5 (Cy5.5) was incorporated into NPs (Cy5.5-NPs). A series of hybrid NPs consisting of NPs, cRGDfK-NPs, and Cy5.5-NPs with drugs encapsulated inside the core (Cy5.5-cRGDfK-NPs/PTX + CUR) were prepared by self-assembly. The efficacy of PTX and CUR combination and the ability of the integrin-mediated targeting of NPs were systemically investigated using a 4T1 mouse breast cancer cell line and a nude mouse xenograft model. We suggested that Cy5.5-cRGDfK-NPs/PTX + CUR has superior theranostic potential against breast carcinoma.

摘要

在这项研究中，制备了整合素介导靶向的近红外荧光（NIRF）可追踪的聚乙二醇-聚（乳酸-共-乙醇酸）（PEG-PLGA）基聚合物纳米颗粒（NPs），以研究紫杉醇（PTX）和姜黄素（CUR）联合治疗对乳腺癌的影响。环（精氨酸-甘氨酸-天冬氨酸-苯丙氨酸-赖氨酸）（cRGDfK）被选为乳腺癌的配体，并连接到 NPs 的末端（cRGDfK-NPs）。为了荧光成像，磺基花菁 5.5（Cy5.5）被掺入 NPs 中（Cy5.5-NPs）。通过自组装制备了一系列包含 NPs、cRGDfK-NPs 和内部封装药物的核（Cy5.5-cRGDfK-NPs/PTX + CUR）的杂化 NPs。使用 4T1 小鼠乳腺癌细胞系和裸鼠异种移植模型系统地研究了 PTX 和 CUR 联合的疗效以及 NPs 整合素介导靶向的能力。我们认为 Cy5.5-cRGDfK-NPs/PTX + CUR 对乳腺癌具有优越的治疗潜力。