MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, China.
State Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
J Proteome Res. 2021 May 7;20(5):2839-2850. doi: 10.1021/acs.jproteome.1c00077. Epub 2021 Apr 19.
The unreasonable misuse of antibiotics has led to the emergence of large-scale drug-resistant bacteria, seriously threatening human health. Compared with drug-sensitive bacteria, resistant bacteria are difficult to clear by host immunity. To fully explore the adaptive mechanism of resistant bacteria to the iron-restricted environment, we performed data-independent acquisition-based quantitative proteomics on ciprofloxacin (CIP)-resistant (CIP-R) in the presence or absence of iron. On bioinformatics analysis, CIP-R bacteria showed stronger amino acid synthesis and energy storage ability. Notably, CIP-R bacteria increased virulence by upregulating the expression of many virulence-related proteins and enhancing the synthesis of virulence-related amino acids under iron-restricted stress. This study will help us to further explain the adaptive mechanisms that lead to bacterial resistance to antibiotics depending on the host environment and provide insights into the development of novel drugs for the treatment of drug-resistant bacterial infections.
抗生素的不合理滥用导致了大规模耐药菌的出现，严重威胁了人类健康。与药敏菌相比，耐药菌更难被宿主免疫清除。为了充分探索耐药菌对缺铁环境的适应机制，我们在有或没有铁的情况下，对环丙沙星（CIP）耐药（CIP-R）进行了基于数据非依赖采集的定量蛋白质组学研究。在生物信息学分析中，CIP-R 细菌表现出更强的氨基酸合成和能量储存能力。值得注意的是，CIP-R 细菌通过上调许多与毒力相关的蛋白质的表达，并在缺铁胁迫下增强与毒力相关的氨基酸合成，从而增加了毒力。这项研究将有助于我们进一步解释导致细菌对抗生素产生耐药性的适应机制，这些机制取决于宿主环境，并为治疗耐药菌感染的新型药物的开发提供思路。
