寡聚α-胸苷酸及其与互补序列相互作用的圆二色性研究。
Circular dichroism studies of an oligo-alpha-thymidylate and of its interactions with complementary sequences.
作者信息
Durand M, Maurizot J C, Thuong N T, Helene C
机构信息
Centre de Biophysique Moleculaire, Orleans, France.
出版信息
Nucleic Acids Res. 1988 Jun 10;16(11):5039-53. doi: 10.1093/nar/16.11.5039.
Abstract
An octathymidylate synthesized with the alpha anomer of thymidine has been studied using circular dichroism. Its conformation has been compared to that of its analogue containing the naturally occurring beta anomer. In both compounds some degree of intramolecular stacking is present as indicated by the shapes of the circular dichroism spectra and their variations with temperature. As its beta-analogue the alpha-octathymidylate binds to its complementary sequences containing beta-nucleosides. Only complexes with 1A:1T stoechiometry were observed. Binding to ribose- containing oligomers and polymers is much stronger than binding to deoxyribose-containing analogues. Circular dichroism spectra provided evidence for a difference between the geometry of the various complexes formed with an alpha-oligothymidylate and those formed with its beta-anomer-containing analogue.
摘要
已使用圆二色性对由胸腺嘧啶核苷的α异头物合成的八聚胸苷酸进行了研究。已将其构象与其含有天然存在的β异头物的类似物的构象进行了比较。圆二色性光谱的形状及其随温度的变化表明，在这两种化合物中都存在一定程度的分子内堆积。α-八聚胸苷酸与其β类似物一样，能与含有β核苷的互补序列结合。仅观察到化学计量比为1A:1T的复合物。与含核糖的寡聚物和聚合物的结合比与含脱氧核糖的类似物的结合要强得多。圆二色性光谱提供了证据，表明由α-寡聚胸苷酸形成的各种复合物的几何形状与由含β异头物的类似物形成的复合物的几何形状之间存在差异。
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