含1-α-D-阿拉伯呋喃糖基胸腺嘧啶的寡核苷酸的制备及杂交特性
Preparation and hybridization properties of oligonucleotides containing 1-alpha-D-arabinofuranosylthymine.
作者信息
Adams A D, Petrie C R, Meyer R B
机构信息
MicroProbe Corporation, Bothell, WA 98021.
出版信息
Nucleic Acids Res. 1991 Jul 11;19(13):3647-51. doi: 10.1093/nar/19.13.3647.
Abstract
A pentadecanucleotide was prepared from 1-alpha-arabinofuranosylthymine. This novel oligonucleotide was found to hybridize to oligodeoxyadenylate, although not a s strongly as pentadecathymidylate. It formed duplex hybrids with both DNA and RNA complements, and triplex structures with a duplex containing a (dT)15-(dA)15 tract within a more complex strand. The Tm of the duplex with polyadenylate was almost the same as that of (dT)15 and polyadenylate, while its Tm with (dA)15 was substantially lower than that of the natural counterpart. A selective benzoylation of the 2'-O of a 5'-blocked alpha-ara-thymine was developed to greatly simplify the preparation of suitable blocked material for use in preparation of oligonucleotides on the automated DNA synthesizer.
摘要
从1-α-阿拉伯呋喃糖基胸腺嘧啶制备了一种十五聚核苷酸。发现这种新型寡核苷酸能与寡聚脱氧腺苷酸杂交，尽管其杂交强度不如十五聚胸苷酸。它能与DNA和RNA互补链形成双链杂交体，并与在更复杂链中含有(dT)15-(dA)15片段的双链形成三链结构。与聚腺苷酸形成的双链的熔解温度与(dT)15和聚腺苷酸的几乎相同，而与(dA)15形成的双链的熔解温度则明显低于天然对应物。开发了一种对5'-封闭的α-阿拉伯糖基胸腺嘧啶的2'-O进行选择性苯甲酰化的方法，以大大简化在自动DNA合成仪上制备用于寡核苷酸制备的合适封闭材料的过程。
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