Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
Plant Physiol Biochem. 2021 Jun;163:276-284. doi: 10.1016/j.plaphy.2021.04.004. Epub 2021 Apr 5.
In plants, although KNOX genes are known to regulate secondary cell wall (SCW) formation, their protein-regulating mechanisms remain largely unknown. Here, we showed that GhKNL1, which regulates SCW formation and fiber development in cotton, could interact with an IQ67 domain containing protein (GhIQD14) in yeast. Confocal observation showed that GhIQD14 was localized to the microtubules. In Arabidopsis, ectopic expression of GhIQD14 caused hypocotyls to be sensitive to microtubule depolymerization agent, organ twisting of seedlings, trichomes, rosette leaves, and capsules, as well as severely irregular xylem vessels and thicker interfascicular fiber cell walls in the inflorescence stem. Furthermore, we found that GhIQD14 interacted with AtKNAT7 in vivo, and instantaneous co-expression of GhIQD14 and AtKNAT7 in tobacco showed that GhIQD14 weakened the distribution of AtKNAT7 in the nucleus, bringing it into the microtubules, thus affecting the SCW formation related genes expression. Our results suggested that GhIQD14 might be involved in the morphological development and SCW formation in cotton.
在植物中，虽然已经知道 KNOX 基因调控次生细胞壁（SCW）的形成，但它们的蛋白质调控机制在很大程度上仍然未知。在这里，我们表明，调控棉花次生细胞壁形成和纤维发育的 GhKNL1 可以在酵母中与一个含有 IQ67 结构域的蛋白（GhIQD14）相互作用。共聚焦观察表明，GhIQD14 定位于微管上。在拟南芥中，异位表达 GhIQD14 导致下胚轴对微管解聚剂敏感，幼苗器官扭曲、毛状体、莲座叶和蒴果，以及花序茎中木质部导管严重不规则和间隔纤维细胞壁变厚。此外，我们发现 GhIQD14 与 AtKNAT7 在体内相互作用，瞬时共表达 GhIQD14 和 AtKNAT7 在烟草中表明，GhIQD14 减弱了 AtKNAT7 在核内的分布，将其带入微管，从而影响与 SCW 形成相关的基因表达。我们的结果表明，GhIQD14 可能参与棉花的形态发育和 SCW 形成。
