Applied Microbiology Lab, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, 110016, India.
Applied Microbiology Lab, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, 110016, India; Department of Biotechnology, Gandhi Institute of Engineering and Technology University, Gunupur, Odisha, 765022, India.
Chemosphere. 2021 Sep;279:130554. doi: 10.1016/j.chemosphere.2021.130554. Epub 2021 Apr 12.
Desizing process in textile industry produces large volume of starch effluent. This carbon-rich waste can be used for resource recovery, such as the production of industrially useful enzymes. The present work assesses the usability of starch effluent from textile industry as an additional carbon source for enhanced production of α-amylase during solid-state fermentation (SSF) of agro-wastes by Trichoderma reesei. A significant increase (p ≤ 0.05) in α-amylase activity (25.48 ± 1.12 U mL) was observed with supplementation of starch effluent in SSF. Partial purification of α-amylase by 80% ammonium sulphate precipitation produced a yield of 58.39% enzyme with purification fold of 1.89. The enzyme was thermally stable at 40 °C with 90% residual activity after 5 h and 70% residual activity at 50 °C after 3 h. Using Michaelis-Menten kinetics analysis, the estimated K and V values for the partially purified α-amylase were found to be 2.55 mg mL and 53.47 U mg, respectively. For the rapid assessment of the industrial application, desizing of the fabric was attempted. The cotton fabric was efficiently desized using α-amylase (at a concentration of 1% on the weight of fabric basis) at 80 °C. The present work demonstrates starch effluent from desizing process as a resource for the production of amylase. The amylase can further be used in the desizing process. With in-depth research, the work may lead to the development of a closed-loop, waste-recycling process for the textile industry.
纺织工业的退浆过程会产生大量的淀粉废水。这种富含碳的废物可以被回收利用，例如生产工业上有用的酶。本研究评估了纺织工业的淀粉废水作为外加碳源，在固态发酵（SSF）中对农业废弃物中里氏木霉（Trichoderma reesei）生产α-淀粉酶的可用性。在 SSF 中添加淀粉废水可显著提高α-淀粉酶活性（25.48±1.12 U mL）（p≤0.05）。通过 80%硫酸铵沉淀对α-淀粉酶进行部分纯化，得到了 58.39%的酶产量，酶的纯化倍数为 1.89。该酶在 40°C 下热稳定性良好，5 h 后仍保持 90%的残余活性，3 h 后在 50°C 下仍保持 70%的残余活性。通过 Michaelis-Menten 动力学分析，部分纯化的α-淀粉酶的估计 K 和 V 值分别为 2.55 mg mL 和 53.47 U mg。为了快速评估其工业应用，尝试对织物进行退浆。在 80°C 下，用浓度为 1%（基于织物重量）的α-淀粉酶可以有效地对棉织物进行退浆。本研究表明，退浆过程中的淀粉废水是生产淀粉酶的一种资源。该酶可以进一步用于退浆过程。随着深入研究，这项工作可能会为纺织工业开发出一个闭环、废物回收的工艺。
