Department of Breast Surgery, Clinical Research Center for Breast, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
Biomed Mater. 2021 Apr 30;16(4). doi: 10.1088/1748-605X/abf982.
Lipofilling is a popular technique for soft tissue augmentation, limited by unpredictable graft survival. This study aimed at exploring the effect of hydrogel from acellular porcine adipose tissue (HAPA) on angiogenesis and survival of adipose tissue used for lipofilling. The effect of HAPA on adipose-derived stem cells (ADSCs) proliferation, adipogenic differentiation, and vascular endothelial growth factor (VEGF) secretion were evaluated in hypoxia and normoxia. For thestudy, adipose tissue with phosphate buffered saline, ADSCs, and HAPA (with or without ADSCs) were co-injected subcutaneously into nude mice. HAPA-ADSCs mixture (tissue engineering adipose tissue) was also grafted. Gross observation, volume measurement, and ultrasound observation were assessed. For histological assessment, hematoxylin and eosin, perilipin, cluster of differentiation 31 (CD31), Ki67, and transferase-mediated d-UTP nick end labelling (TUNEL) staining were performed. HAPA improved ADSCs proliferation, VEGF secretion, and adipogenic differentiation under normoxia and hypoxia conditionsstudy. For thestudy, HAPA showed improved volume retention and angiogenesis, and reduced cell apoptosis when compared to ADSCs-assisted lipofilling and pure lipofilling. In conclusion, HAPA could maintain ADSCs viability and improve cell resistant to hypoxia and might be a promising biomaterial to assist lipofilling.
脂肪填充是一种用于软组织填充的热门技术，但存在移植物存活率不可预测的局限性。本研究旨在探索脱细胞猪脂肪组织水凝胶（HAPA）对脂肪填充用脂肪组织血管生成和存活的影响。在低氧和常氧条件下，评估了 HAPA 对脂肪来源干细胞（ADSCs）增殖、成脂分化和血管内皮生长因子（VEGF）分泌的影响。在该研究中，将磷酸盐缓冲盐水、ADSCs 和 HAPA（有或没有 ADSCs）与脂肪组织一起注射到裸鼠的皮下。还移植了 HAPA-ADSCs 混合物（组织工程脂肪组织）。进行大体观察、体积测量和超声观察。为了进行组织学评估，进行了苏木精和伊红、脂联素、分化群 31（CD31）、Ki67 和转移酶介导的 d-UTP 缺口末端标记（TUNEL）染色。在常氧和低氧条件下，HAPA 可促进 ADSCs 的增殖、VEGF 分泌和成脂分化。与 ADSCs 辅助脂肪填充和单纯脂肪填充相比，HAPA 显示出改善的体积保留和血管生成，以及减少的细胞凋亡。总之，HAPA 可以维持 ADSCs 的活力并提高细胞对缺氧的抵抗力，可能是一种有前途的辅助脂肪填充的生物材料。
