Department of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Department of Plastic and Reconstructive Surgery, the First Medical Center, Chinese PLA (People's Liberation Army) General Hospital, Beijing, China.
J Appl Biomater Funct Mater. 2021 Jan-Dec;19:2280800020988141. doi: 10.1177/2280800020988141.
Adipose-derived stem cells (ADSCs) have been widely researched and used as a drug therapy in many fields like disease treatment and tissue engineering. However, ADSCs are susceptible to the surrounding environment. The emergence of acellular extracellular matrix provides a solution, which can serve as biomaterial scaffold as well as original ecological niche for the stem cells. Therefore, we propose the hypothesis that human decellularized adipose tissues (hDAT) are processed into injectable hydrogels and then mixed evenly with ADSCs. So that the ADSCs embedded-hydrogels could directly carry the stem cells to the appropriate sites. The hDAT hydrogel could provide microenvironmental protection for ADSCs. In this study, we successfully made human decellularized adipose tissue hydrogel (hDAT-gel), which was temperature-sensitive, liquid at 4°C and semi-solid at 37°C. When the ADSCs were embedded in hDAT-gel, they survived well and continued to grow well in layers. When the pre-gel containing ADSCs was injected subcutaneously into nude mice, the sample results after 15 min showed gelation occurred in situ. These results suggested that hDAT-gel could provide a culture platform for ADSCs delivery.
脂肪来源干细胞（ADSCs）已在许多领域（如疾病治疗和组织工程）被广泛研究和用作药物治疗。然而，ADSCs 易受周围环境影响。无细胞细胞外基质的出现提供了一种解决方案，它可以作为生物材料支架以及干细胞的原始生态位。因此，我们提出了一个假设，即人类去细胞脂肪组织（hDAT）被加工成可注射水凝胶，然后与 ADSCs 均匀混合。这样，嵌入水凝胶中的 ADSCs 可以直接将干细胞输送到合适的部位。hDAT 水凝胶可以为 ADSCs 提供微环境保护。在这项研究中，我们成功地制备了人源去细胞脂肪组织水凝胶（hDAT-gel），其具有温度敏感性，在 4°C 时为液体，在 37°C 时为半固体。当 ADSCs 嵌入 hDAT-gel 中时，它们在各层中存活良好并继续良好生长。当含有 ADSCs 的预凝胶被皮下注射到裸鼠中时，15 分钟后的样本结果显示原位发生凝胶化。这些结果表明 hDAT-gel 可以为 ADSCs 的输送提供一个培养平台。
